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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-4-19
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pubmed:abstractText |
Staurosporine is a potent apoptosis inducer, but its mechanism remains to be clarified. We investigated the involvement of PTEN in staurosporine-induced apoptosis. Ishikawa cells, from an endometrial carcinoma cell line, expressed a high amount of PTEN mRNA but did not express the PTEN protein because of protein truncations. We isolated clones expressing the steady-state level of the PTEN protein from PTEN-null Ishikawa cells by transfection. The obtained clones showed reduced proliferative activity and reduced anchorage-independent cell growth with the augmented p27(Kip1). These cell lines were sensitized to apoptosis by staurosporine. A low concentration of UCN-01 did not affect apoptosis, but a high concentration augmented apoptosis in the PTEN-expressing clone. Alpha-sphingosine and H-7 did not affect apoptosis in these cell lines. PI3K inhibition augmented staurosporine-induced apoptosis in the parental cell line, but not in the PTEN-expressing clone. In the clone, phosho-Akt/PKB and phospho-Bad (Ser-136) were downregulated. Staurosporine reduced the levels of phospho-Akt/PKB and phospho-Bad (Ser-136) in all the cell lines, but the reduction was most significant in the PTEN-expressing clone. These results suggest that inhibition of the PI3K/Akt/PKB signaling pathway might be associated with staurosporine-induced apoptosis in Ishikawa cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1350-9047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
414-20
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11965494-Animals,
pubmed-meshheading:11965494-Antineoplastic Agents,
pubmed-meshheading:11965494-Apoptosis,
pubmed-meshheading:11965494-Carcinoma,
pubmed-meshheading:11965494-Cells, Cultured,
pubmed-meshheading:11965494-Clone Cells,
pubmed-meshheading:11965494-Down-Regulation,
pubmed-meshheading:11965494-Endometrial Neoplasms,
pubmed-meshheading:11965494-Female,
pubmed-meshheading:11965494-Mutation,
pubmed-meshheading:11965494-PTEN Phosphohydrolase,
pubmed-meshheading:11965494-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11965494-Phosphoric Monoester Hydrolases,
pubmed-meshheading:11965494-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11965494-Proto-Oncogene Proteins,
pubmed-meshheading:11965494-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:11965494-Signal Transduction,
pubmed-meshheading:11965494-Staurosporine,
pubmed-meshheading:11965494-Transfection,
pubmed-meshheading:11965494-Tumor Cells, Cultured,
pubmed-meshheading:11965494-Tumor Suppressor Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
PTEN augments staurosporine-induced apoptosis in PTEN-null Ishikawa cells by downregulating PI3K/Akt signaling pathway.
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pubmed:affiliation |
Department of Obstetrics and Gynecology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu, 500-8705, Japan.
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pubmed:publicationType |
Journal Article
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