Source:http://linkedlifedata.com/resource/pubmed/id/11957146
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2002-4-16
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| pubmed:abstractText |
Mutations in the beta-catenin gene (CTNNB 1) with abnormal nuclear accumulation of beta-catenin have recently been identified in endometrial carcinoma (EC). Their relationship with microsatellite instability (MI) is unclear. It has been suggested that matrix metalloproteinase-7 (MMP-7) and cyclin D1 (cD) genes are targets for beta-catenin activation. DNA from 73 patients with EC was obtained from tumor and normal tissue (59 endometrioid and 14 nonendometrioid). CTNNB 1 mutations in exon 3 were assessed by single-strand conformation polymorphism and DNA sequencing. The results were correlated with immunostaining for beta-catenin, MMP-7, and cD. Three (CA)n repeats and mononucleotide tracts BAT 25 and BAT 26 had been previously used for MI analysis. CTNNB1 mutations were identified in 15 ECs (20.5%), all of them endometrioid carcinomas (15 of 59; 25.4%). They occurred in 6 of 19 MI-positive ECs (31.5%) and in 9 of 54 MI-negative ECs (16.6%). Eleven of the 15 CTNNB 1-mutated ECs showed beta-catenin nuclear immunostaining (P <.05). MMP-7 expression (>50% cells) was observed in 23 ECs, with 7 of these showing CTNNB 1 mutations. Significant expression of cD (>50% cells) was detected in 8 ECs, with 5 of these exhibiting CTNNB 1 mutations (P <.05). The results confirm that beta-catenin plays a role in endometrial carcinogenesis, particularly in endometrioid carcinomas. The results also suggest that MMP-7 and particularly cD may be targets of beta-catenin activation in ECs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 7,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0046-8177
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002, Elsevier Science (USA). All rights reserved.
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| pubmed:issnType |
Print
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| pubmed:volume |
33
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
206-12
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11957146-Adenocarcinoma,
pubmed-meshheading:11957146-Cell Nucleus,
pubmed-meshheading:11957146-Cyclin D1,
pubmed-meshheading:11957146-Cytoskeletal Proteins,
pubmed-meshheading:11957146-DNA, Neoplasm,
pubmed-meshheading:11957146-DNA Mutational Analysis,
pubmed-meshheading:11957146-Endometrial Neoplasms,
pubmed-meshheading:11957146-Exons,
pubmed-meshheading:11957146-Female,
pubmed-meshheading:11957146-Gene Expression,
pubmed-meshheading:11957146-Humans,
pubmed-meshheading:11957146-Immunohistochemistry,
pubmed-meshheading:11957146-Matrix Metalloproteinase 7,
pubmed-meshheading:11957146-Mutation,
pubmed-meshheading:11957146-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:11957146-Sequence Analysis, DNA,
pubmed-meshheading:11957146-Trans-Activators,
pubmed-meshheading:11957146-beta Catenin
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| pubmed:year |
2002
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| pubmed:articleTitle |
CTNNB1 mutations and beta-catenin expression in endometrial carcinomas.
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| pubmed:affiliation |
Department of Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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