Source:http://linkedlifedata.com/resource/pubmed/id/11955497
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-4-16
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pubmed:abstractText |
In cell stress research, there is still a need to apply long-term hydrostatic pressure without changing any other environmental condition. We present here a new, open, pressurized chamber system allowing long-term sustained and dynamic application of hydrostatic pressure with the option of additional tension. Based on the computer-controlled Flexcell Strain Unit, we designed a pressurized chamber with a dynamic airflow and a defined membrane extension, which can be regulated by spacers. During operation up to 26.6kPa, O(2) partial pressures and pH in the cell-culture medium do not change compared to control cultures kept at normal atmosphere.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9290
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
579-84
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pubmed:dateRevised |
2009-11-11
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pubmed:meshHeading |
pubmed-meshheading:11955497-Cell Culture Techniques,
pubmed-meshheading:11955497-Cytological Techniques,
pubmed-meshheading:11955497-Equipment Design,
pubmed-meshheading:11955497-Humans,
pubmed-meshheading:11955497-Hydrogen-Ion Concentration,
pubmed-meshheading:11955497-Hydrostatic Pressure,
pubmed-meshheading:11955497-Muscle, Smooth, Vascular,
pubmed-meshheading:11955497-Oxygen,
pubmed-meshheading:11955497-Partial Pressure,
pubmed-meshheading:11955497-Stress, Physiological,
pubmed-meshheading:11955497-Surface Tension
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pubmed:year |
2002
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pubmed:articleTitle |
A cell-culture system for long-term maintenance of elevated hydrostatic pressure with the option of additional tension.
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pubmed:affiliation |
Department of Pathology, Ulm University, Albert-Einstein-Allee 11, D-89089 Ulm, Germany.
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pubmed:publicationType |
Journal Article
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