| pubmed-article:11943205 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0740009 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0001830 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0766823 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0972255 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11943205 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11943205 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:11943205 | pubmed:dateCreated | 2002-4-10 | lld:pubmed |
| pubmed-article:11943205 | pubmed:abstractText | Potential contamination of animal-derived collagen with pathogens has led to the demand for safe recombinant sources of this complex molecule. In continuation of our previous work [Ruggiero et al. (2000) FEBS Lett. 469, 132-136], here we show that it is possible to produce recombinant hydroxylated homotrimeric collagen in tobacco plants that are co-transformed with a human type I collagen and a chimeric proline-4-hydroxylase (P4H). This is to our knowledge the first time that transient expression in tobacco was used to improve the quality of a recombinant protein produced in plants through co-expression with an animal cell-derived modifying enzyme. We demonstrated the functionality of the new chimeric P4H and thus improved the thermal stability of recombinant collagen I from plants to 37 degrees C. | lld:pubmed |
| pubmed-article:11943205 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11943205 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11943205 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11943205 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11943205 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:GarroneRR | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:MerloMM | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:TheisenMM | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:RuggieroFF | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:LacourTT | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:PerretSS | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:JonvalVV | lld:pubmed |
| pubmed-article:11943205 | pubmed:author | pubmed-author:HudaverdianSS | lld:pubmed |
| pubmed-article:11943205 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11943205 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:11943205 | pubmed:volume | 515 | lld:pubmed |
| pubmed-article:11943205 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11943205 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11943205 | pubmed:pagination | 114-8 | lld:pubmed |
| pubmed-article:11943205 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11943205 | pubmed:meshHeading | pubmed-meshheading:11943205... | lld:pubmed |
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| pubmed-article:11943205 | pubmed:meshHeading | pubmed-meshheading:11943205... | lld:pubmed |
| pubmed-article:11943205 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11943205 | pubmed:articleTitle | Hydroxylated human homotrimeric collagen I in Agrobacterium tumefaciens-mediated transient expression and in transgenic tobacco plant. | lld:pubmed |
| pubmed-article:11943205 | pubmed:affiliation | Meristem Therapeutics, 8 rue des Frères Lumière, 63100, Clermont-Ferrand, France. | lld:pubmed |
| pubmed-article:11943205 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11943205 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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