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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2002-4-10
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pubmed:abstractText |
Potential contamination of animal-derived collagen with pathogens has led to the demand for safe recombinant sources of this complex molecule. In continuation of our previous work [Ruggiero et al. (2000) FEBS Lett. 469, 132-136], here we show that it is possible to produce recombinant hydroxylated homotrimeric collagen in tobacco plants that are co-transformed with a human type I collagen and a chimeric proline-4-hydroxylase (P4H). This is to our knowledge the first time that transient expression in tobacco was used to improve the quality of a recombinant protein produced in plants through co-expression with an animal cell-derived modifying enzyme. We demonstrated the functionality of the new chimeric P4H and thus improved the thermal stability of recombinant collagen I from plants to 37 degrees C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
515
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
114-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11943205-Agrobacterium tumefaciens,
pubmed-meshheading:11943205-Amino Acids,
pubmed-meshheading:11943205-Biological Assay,
pubmed-meshheading:11943205-Chromatography, High Pressure Liquid,
pubmed-meshheading:11943205-Circular Dichroism,
pubmed-meshheading:11943205-Collagen Type I,
pubmed-meshheading:11943205-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11943205-Gene Expression,
pubmed-meshheading:11943205-Humans,
pubmed-meshheading:11943205-Hydroxylation,
pubmed-meshheading:11943205-Pepsin A,
pubmed-meshheading:11943205-Plants, Genetically Modified,
pubmed-meshheading:11943205-Procollagen-Proline Dioxygenase,
pubmed-meshheading:11943205-Protein Conformation,
pubmed-meshheading:11943205-Recombinant Fusion Proteins,
pubmed-meshheading:11943205-Recombinant Proteins,
pubmed-meshheading:11943205-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11943205-Temperature,
pubmed-meshheading:11943205-Tobacco,
pubmed-meshheading:11943205-Transformation, Genetic
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pubmed:year |
2002
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pubmed:articleTitle |
Hydroxylated human homotrimeric collagen I in Agrobacterium tumefaciens-mediated transient expression and in transgenic tobacco plant.
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pubmed:affiliation |
Meristem Therapeutics, 8 rue des Frères Lumière, 63100, Clermont-Ferrand, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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