Source:http://linkedlifedata.com/resource/pubmed/id/11931160
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 2
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pubmed:dateCreated |
2002-4-4
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pubmed:databankReference | |
pubmed:abstractText |
Five brightly red-pigmented, motile, rod-shaped, extremely halophilic bacteria were isolated from saltern crystallizer ponds in Alicante (two strains) and Mallorca (three strains), Spain. They grew optimally at salt concentrations between 20 and 30% and did not grow below 15% salts. Thus, these isolates are among the most halophilic organisms known within the domain Bacteria. The temperature optimum was 37-47 degrees C. A single, yet to be identified pigment was present, with an absorption maximum at 482 nm and a shoulder at 506-510 nm. The G+C content of the DNA was 66.3-67.7 mol% and, together, they formed a homogeneous genomic group with DNA-DNA similarities above 70%. The 16S rRNA gene sequences were almost identical to sequences recovered earlier from the saltern biomass by amplification of bacterial small-subunit rRNA genes from DNA extracted from the environment. This phylotype, earlier described as 'Candidatus Salinibacter', was shown by fluorescence in situ hybridization to contribute between 5 and 25% of the prokaryote community of the saltern crystallizers. We have therefore succeeded in isolating a bacterium from the natural environment that, although being a major component of the community, was previously known by its phylotype only. Isolation of the organism now allows formal description of a novel genus and species, for which we propose the name Salinibacter ruber gen. nov., sp. nov. The type strain is strain M31T (= DSM 13855T = CECT 5946T).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Pigments, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1466-5026
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11931160-Bacteria,
pubmed-meshheading:11931160-Bacterial Physiological Phenomena,
pubmed-meshheading:11931160-Base Composition,
pubmed-meshheading:11931160-DNA, Bacterial,
pubmed-meshheading:11931160-Fresh Water,
pubmed-meshheading:11931160-Molecular Sequence Data,
pubmed-meshheading:11931160-Phylogeny,
pubmed-meshheading:11931160-Pigments, Biological,
pubmed-meshheading:11931160-RNA, Ribosomal,
pubmed-meshheading:11931160-RNA, Ribosomal, 16S,
pubmed-meshheading:11931160-Sequence Homology, Nucleic Acid,
pubmed-meshheading:11931160-Sodium Chloride,
pubmed-meshheading:11931160-Spain,
pubmed-meshheading:11931160-Species Specificity,
pubmed-meshheading:11931160-Temperature
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pubmed:year |
2002
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pubmed:articleTitle |
Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds.
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pubmed:affiliation |
Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Spain. anton@ua.es
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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