Linked Life Data

11913780

Source:http://linkedlifedata.com/resource/pubmed/id/11913780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2002-3-26
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB041264, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042402, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042845, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042846, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042847, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042848, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042849, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042850, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042851, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042852, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042853, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB042854, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB045007, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB045718, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB051388
pubmed:abstractText
The functional significance of heterotrimeric guanine nucleotide binding protein (G protein) for the many physiological processes including the molecular mechanisms of drug addiction have been described. In investigating the changes of mRNA expression after acute psychostimulant administration, we previously identified a cDNA encoding a G protein beta1 subunit (Gbeta1) that was increased up to four-fold in certain brain regions after administration of psychostimulants. The mouse Gbeta1 gene (the mouse genetic symbol, GNB1) was mapped to chromosome 4, but little was known of its genetic features. To characterize the GNB1 gene further, we have cloned and analyzed the genomic structures of the mouse GNBI gene and its homologous sequences. The GNBI gene spans at least 50 kb, and consists of 12 exons and 11 introns. The exon/intron boundaries were determined and found to follow the GT/AG rule. Exons 3-11 encode the Gbeta1 protein, and the exon 2 is an alternative, resulting in putative two splicing variants. Although intron 11 is additional for GNBI compared with GNB2 and GNB3, the intron positions within the protein coding region of GNB1, GNB2 and GNB3 are identical, suggesting that GNB1 should have diverged from the ancestral gene family earlier than the genes for GNB2 and GNB3. We also found the 5'-truncated processed pseudogenes with 71-89% similarities to GNBI mRNA sequence, suggesting that the truncated cDNA copies, which have been reverse-transcribed from a processed mRNA for GNB1, might have been integrated into several new locations in the mouse genome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1042-5179
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345-54
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Genomic organization of the murine G protein beta subunit genes and related processed pseudogenes.
pubmed:affiliation
Molecular Neurobiology Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. kitanaka@hyogomc.kugi.kyoto-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.