Linked Life Data

11906929

Source:http://linkedlifedata.com/resource/pubmed/id/11906929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-3-21
pubmed:abstractText
Members of the matrix metalloproteinase (MMP) family collectively degrade extracellular matrix (ECM) and help regulate luteal function. The objectives of these experiments were to characterize the mRNA expression, localization, and activity of MMPs 2, 9, and 14 in ovine corpora lutea (CL). Ovine CL were collected on Days 2, 4, 10, and 15 of the estrous cycle (Day 0 = estrus). Messenger RNA transcripts for MMPs 2 and 14 were detected using Northern analysis; however, expression of MMP-9 was undetectable. Expression of MMP-14 mRNA (membrane type-1 MMP) was increased (P < 0.05) on Day 4; whereas, expression of MMP-2 mRNA was highest (P < 0.05) on Day 10, which corresponded to the observed increases in gelatinolytic activity in luteal homogenates as measured by a fluroscein-labeled gelatin substrate assay. MMP 2 and 9 proteins were localized predominantly to large luteal cells (LLCs), whereas MMP-14 was localized primarily to cells other than LLCs as demonstrated by immunohistochemistry. Immunolocalization of MMP-2 to putative endothelial cells was also observed on Day 15. Localization of MMP activity was determined using in situ zymography. Luteal tissues contained gelatinolytic activity primarily localized pericellularly to various cell types, including LLCs. These results support the hypothesis that ECM remodeling occurs throughout the luteal phase and may help potentiate cellular migration, differentiation, angiogenesis, and growth factor bioavailability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1083-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11906929-Animals, pubmed-meshheading:11906929-Blotting, Northern, pubmed-meshheading:11906929-Corpus Luteum, pubmed-meshheading:11906929-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11906929-Estrous Cycle, pubmed-meshheading:11906929-Female, pubmed-meshheading:11906929-Fluorescein, pubmed-meshheading:11906929-Fluorescent Dyes, pubmed-meshheading:11906929-Gelatin, pubmed-meshheading:11906929-Immunohistochemistry, pubmed-meshheading:11906929-Matrix Metalloproteinase 2, pubmed-meshheading:11906929-Matrix Metalloproteinase 9, pubmed-meshheading:11906929-Matrix Metalloproteinases, Membrane-Associated, pubmed-meshheading:11906929-Metalloendopeptidases, pubmed-meshheading:11906929-Progesterone, pubmed-meshheading:11906929-RNA, Messenger, pubmed-meshheading:11906929-Sheep
pubmed:year
2002
pubmed:articleTitle
Matrix metalloproteinase (2, 9, and 14) expression, localization, and activity in ovine corpora lutea throughout the estrous cycle.
pubmed:affiliation
Department of Animal Science, 160 Animal Sciences Center, University of Missouri, Columbia, MO 65211, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.