Linked Life Data

11891846

Source:http://linkedlifedata.com/resource/pubmed/id/11891846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-3-13
pubmed:abstractText
We have previously reported that glucocorticoids markedly increase and anti-glucocorticoids (such as RU-486) block c-fms RNA and protein expression in some breast cancer cell lines, but not in others, and that this increase is the consequence of increased transcription from the first, epithelial cell-specific promoter of the c-fms gene (encoding CSF-1R, macrophage colony-stimulating factor receptor). Employing DNaseI protection and electrophoretic mobility shift assays (EMSA), we now demonstrate that DNA-transcription factor protein complexes are formed on the c-fms first promoter at a composite regulatory element containing overlapping binding sites for AP-1 proteins, bHLH factors, and the glucocorticoid receptor (GR). Competition studies indicate that transcription factor proteins bind the AP-1 site and the GR element (GRE) and both GR and AP-1 proteins are involved in DNA-protein complex formation. The complexes differ in quantity and glucocorticoid inducibility in the different breast cancer cell lines studied depending on whether the promoter responds to glucocorticoid stimulation. Transient transfection of promoter/reporter gene constructs resulted in reduced basal transcription activity of this promoter and lack of glucocorticoid stimulation when the AP-1 site was mutated. We conclude that AP-1 proteins, GR and associated co-factors regulate transcription from the c-fms first promoter and that differences in recruitment of the various components are responsible for cell specific repression and activation of this gene in breast carcinoma cell lines.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-23
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11891846-Antibodies, pubmed-meshheading:11891846-Base Sequence, pubmed-meshheading:11891846-Binding, Competitive, pubmed-meshheading:11891846-Breast Neoplasms, pubmed-meshheading:11891846-Consensus Sequence, pubmed-meshheading:11891846-DNA Probes, pubmed-meshheading:11891846-Deoxyribonuclease I, pubmed-meshheading:11891846-Dexamethasone, pubmed-meshheading:11891846-Electrophoretic Mobility Shift Assay, pubmed-meshheading:11891846-Epithelial Cells, pubmed-meshheading:11891846-Gene Expression Regulation, Neoplastic, pubmed-meshheading:11891846-Genes, Regulator, pubmed-meshheading:11891846-Genes, Reporter, pubmed-meshheading:11891846-Genes, fms, pubmed-meshheading:11891846-Glucocorticoids, pubmed-meshheading:11891846-Humans, pubmed-meshheading:11891846-Molecular Sequence Data, pubmed-meshheading:11891846-Mutation, pubmed-meshheading:11891846-Promoter Regions, Genetic, pubmed-meshheading:11891846-Receptor, Macrophage Colony-Stimulating Factor, pubmed-meshheading:11891846-Transcription, Genetic, pubmed-meshheading:11891846-Transcription Factor AP-1, pubmed-meshheading:11891846-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Hormonal regulation of the c-fms proto-oncogene in breast cancer cells is mediated by a composite glucocorticoid response element.
pubmed:affiliation
Department of Therapeutic Radiology, Yale University School of Medicine, 333 Cedar St, New Haven, Connecticut 06510-8040, USA. maryann.flick@yale.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't