Source:http://linkedlifedata.com/resource/pubmed/id/11881808
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-3-7
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pubmed:databankReference | |
pubmed:abstractText |
This paper describes cDNAs of four small-size proteins that occur in the cocoon silk of Bombyxmori. Two of them (9.9 and 10.3 kDa), which have the N-terminal sequences and the spacing of a few amino acids at C-termini similar to the seroin of Galleria mellonella, are called seroin 1 and seroin 2. The corresponding genes are expressed in the middle, and to a small extent also in the posterior silk gland sections. The seroin 1 and less conspicuously the seroin 2 mRNAs accumulate in the course of the last larval instar to a maximum in postspinning larvae. Two other proteins (6 kDa and 4.7 kDa) of B. mori cocoons were identified as a typical Kunitz-type and a somewhat unusual Kazal-type proteinase inhibitors, and named BmSPI 1 and BmSPI 2, respectively. Their genes are expressed in the middle, and the BmSPI 1 gene slightly also in the posterior silk gland sections. The expression ensues a few days after the last larval ecdysis and increases until the cocoon spinning. Post-spinning larvae still contain high amounts of the BmSPI 1 but no BmSPI 2 transcripts. It is assumed that seroins and proteinase inhibitors are involved in cocoon protection against predators and microbes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insect Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protease Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Seroin protein, Galleria mellonella,
http://linkedlifedata.com/resource/pubmed/chemical/Silk
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0962-1075
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
437-45
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pubmed:dateRevised |
2009-11-3
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pubmed:meshHeading |
pubmed-meshheading:11881808-Amino Acid Sequence,
pubmed-meshheading:11881808-Animals,
pubmed-meshheading:11881808-Base Sequence,
pubmed-meshheading:11881808-Blotting, Northern,
pubmed-meshheading:11881808-Bombyx,
pubmed-meshheading:11881808-DNA, Complementary,
pubmed-meshheading:11881808-Databases, Protein,
pubmed-meshheading:11881808-Expressed Sequence Tags,
pubmed-meshheading:11881808-Glycoproteins,
pubmed-meshheading:11881808-Insect Proteins,
pubmed-meshheading:11881808-Molecular Sequence Data,
pubmed-meshheading:11881808-Protease Inhibitors,
pubmed-meshheading:11881808-Sequence Alignment,
pubmed-meshheading:11881808-Sequence Homology, Amino Acid,
pubmed-meshheading:11881808-Silk
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pubmed:year |
2001
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pubmed:articleTitle |
Identification of four small molecular mass proteins in the silk of Bombyx mori.
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pubmed:affiliation |
Institute of Entomology, Academy of Sciences, and Faculty of Biological Sciences, University of South Bohemia, Ceské Budejovice, Czech Republic.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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