Source:http://linkedlifedata.com/resource/pubmed/id/11875254
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4 Suppl
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pubmed:dateCreated |
2002-3-4
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pubmed:abstractText |
Carbonic anhydrases are a widely expressed family of enzymes that catalyze the reversible reaction: CO(2) + H(2)O <=> HCO(3)(-) + H(+). These enzymes therefore both produce HCO(3)(-) for transport across membranes and consume HCO(3)(-) that has been transported across membranes. Thus these enzymes could be expected to have a key role in driving the transport of HCO(3)(-) across cells and epithelial layers. Plasma membrane anion exchange proteins (AE) transport chloride and bicarbonate across most mammalian membranes in a one-for-one exchange reaction and act as a model for our understanding of HCO(3)(-) transport processes. Recently it was shown that AE1, found in erythrocytes and kidney, binds carbonic anhydrase II (CAII) via the cytosolic C-terminal tail of AE1. To examine the physiological consequences of the interaction between CAII and AE1, we characterized Cl(-)/HCO(3)(-) exchange activity in transfected HEK293 cells. Treatment of AE1-transfected cells with acetazolamide, a CAII inhibitor, almost fully inhibited anion exchange activity, indicating that endogenous CAII activity is essential for transport. Further experiments to examine the role of the AE1/CAII interaction will include measurements of the transport activity of AE1 following mutation of the CAII binding site. In a second approach a functionally inactive CA mutant, V143Y, will be co-expressed with AE1 in HEK293 cells. Since over expression of V143Y CAII would displace endogenous wild-type CAII from AE1, a loss of transport activity would be observed if binding to the AE1 C-terminus is required for transport.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1590-8577
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading | |
pubmed:year |
2001
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pubmed:articleTitle |
Carbonic anhydrase: in the driver's seat for bicarbonate transport.
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pubmed:affiliation |
Membrane Transport Group and CIHR Group in Molecular Biology of Membrane Proteins, Department of Physiology, University of Alberta. Edmonton, Canada.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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