Linked Life Data

11870083

Source:http://linkedlifedata.com/resource/pubmed/id/11870083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-2-28
pubmed:abstractText
Fas antigen (Fas) is a cell surface receptor that triggers apoptosis in sensitive cells when bound to the Fas ligand (Fas L). The present study was undertaken to identify the presence of a Fas-Fas L system in bovine corpus luteum (CL) and to evaluate the regulation of Fas-mediated luteal cell death by leukocyte-derived cytokines. The reverse transcription-polymerase chain reaction showed higher levels of Fas mRNA expression in CL in the regressed luteal stage (Days 19-21) than in the other stages (P < 0.05). Bovine luteal cells from midcycle CL (Days 8-12) were exposed for 24 h to interferon gamma (IFN; 50 ng/ml) and/or tumor necrosis factor alpha (TNF; 50 ng/ml). After 24 h of culture, the expression of Fas mRNA was detected in the cultured cells and was increased by IFN. Moreover, TNF augmented the stimulatory action of IFN, whereas TNF alone did not affect the expression of Fas mRNA. The effects of IFN and TNF on Fas-mediated cell death were also examined. Cells were exposed to IFN and/or TNF for 24 h and were further treated with IFN and/or TNF in the presence or absence of Fas L (100 ng/ml) for 24 h. Treatments of the cells with IFN alone and in combination with TNF resulted in killing of 30% and 50% of the cells (P < 0.05), respectively, whereas TNF alone did not have a cytotoxic effect on the cells. On the other hand, Fas L killed 60% of the cells treated with IFN (P < 0.01) and 85% of the cells treated with the combination of TNF and IFN (P < 0.01), respectively, whereas Fas L showed no effect on the viability of the luteal cells treated with or without TNF. Furthermore, shrunken nuclei and apoptotic bodies were observed in the cells treated with Fas L in the presence of TNF and IFN. The overall results suggest that a Fas-Fas L system is present in bovine CL and that leukocyte-derived TNF and IFN play important roles in Fas-mediated luteal cell death.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
754-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11870083-Animals, pubmed-meshheading:11870083-Antigens, CD95, pubmed-meshheading:11870083-Apoptosis, pubmed-meshheading:11870083-Cattle, pubmed-meshheading:11870083-Cells, Cultured, pubmed-meshheading:11870083-Corpus Luteum, pubmed-meshheading:11870083-Cytokines, pubmed-meshheading:11870083-Fas Ligand Protein, pubmed-meshheading:11870083-Female, pubmed-meshheading:11870083-Gene Expression, pubmed-meshheading:11870083-Humans, pubmed-meshheading:11870083-In Situ Nick-End Labeling, pubmed-meshheading:11870083-Membrane Glycoproteins, pubmed-meshheading:11870083-Propidium, pubmed-meshheading:11870083-RNA, Messenger, pubmed-meshheading:11870083-Recombinant Proteins, pubmed-meshheading:11870083-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11870083-Tumor Necrosis Factor-alpha
pubmed:year
2002
pubmed:articleTitle
Fas-Fas ligand system mediates luteal cell death in bovine corpus luteum.
pubmed:affiliation
Laboratory of Reproductive Endocrinology, Department of Animal Science, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't