Source:http://linkedlifedata.com/resource/pubmed/id/11860489
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2002-2-25
|
| pubmed:abstractText |
Cochlear outer hair cells (OHCs) possess a unique fast voltage-driven motility associated with a voltage-sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin-dependent mechanical pathway which involves a submembranous circumferential cytoskeleton. The purpose of this study was to compare the functional appearance of the voltage-sensitive motor proteins with that involving the Ca2+-sensitive cytoskeleton during postnatal development of rat OHCs. We demonstrate that whole-cell electromotility and Ca2+-voked mechanical responses, by ionomycin, develop concomitantly after postnatal day 5 (P5). These two mechanical properties also develop simultaneously in OHCs isolated from two-week-old cultures of P0-P1 organs of Corti. This excludes the participation of neural innervation in the postnatal maturation of the OHCs' motile properties. In addition, we show that the expression of the membranous voltage-sensitive motor protein precedes, by several days, the appearance of whole-cell electromotility. The concomitant development of whole-cell electromotility and Ca2+-sensitive motility, both in vivo and in vitro, underlines the cytoskeleton as an important factor in the functional organization of the voltage-sensitive motor proteins within the plasma membrane.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0953-816X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1947-52
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| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11860489-Aging,
pubmed-meshheading:11860489-Animals,
pubmed-meshheading:11860489-Animals, Newborn,
pubmed-meshheading:11860489-Calcium Signaling,
pubmed-meshheading:11860489-Cell Differentiation,
pubmed-meshheading:11860489-Cell Membrane,
pubmed-meshheading:11860489-Cell Movement,
pubmed-meshheading:11860489-Cytoskeleton,
pubmed-meshheading:11860489-Electric Stimulation,
pubmed-meshheading:11860489-Hair Cells, Auditory, Outer,
pubmed-meshheading:11860489-Hearing,
pubmed-meshheading:11860489-Ion Channels,
pubmed-meshheading:11860489-Ionomycin,
pubmed-meshheading:11860489-Ionophores,
pubmed-meshheading:11860489-Membrane Potentials,
pubmed-meshheading:11860489-Molecular Motor Proteins,
pubmed-meshheading:11860489-Organ Culture Techniques,
pubmed-meshheading:11860489-Rats,
pubmed-meshheading:11860489-Rats, Wistar,
pubmed-meshheading:11860489-Signal Transduction
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells.
|
| pubmed:affiliation |
Laboratoire de Biologie Cellulaire et Moléculaire de l'Audition, EMI INSERM 99-27, Université de Bordeaux 2, Hôpital Pellegrin, Bat PQR, 33076 Bordeaux, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|