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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-2-20
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pubmed:abstractText |
Recombinant protein rNS3 imitating helicase region (1356-1459 amino acid residues) of hepatitis C virus (HCV) was expressed in E. coli cells and used for BALB/c mice immunization. Seven hybrydoma clones producing monoclonal antibodies (MAbs) to rHS3 were obtained. All MAbs reacted in ELISA with NS3 protein from Murex anti-HCV Version III and in immunoblotting from RIBA 3. These MAbs detect 5 individual epitopes, 4 of which were conformational and 1 discontinuous. All MAbs could compete for rNS3 binding with serum antibodies from patients with chronic hepatitis C, which suggests that these MAbs can recognize the natural HCV NS3 protein.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0507-4088
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11852778-Animals,
pubmed-meshheading:11852778-Antibodies, Monoclonal,
pubmed-meshheading:11852778-Binding, Competitive,
pubmed-meshheading:11852778-Epitopes,
pubmed-meshheading:11852778-Escherichia coli,
pubmed-meshheading:11852778-Genetic Vectors,
pubmed-meshheading:11852778-Hepatitis C, Chronic,
pubmed-meshheading:11852778-Hepatitis C Antibodies,
pubmed-meshheading:11852778-Humans,
pubmed-meshheading:11852778-Mice,
pubmed-meshheading:11852778-Mice, Inbred BALB C,
pubmed-meshheading:11852778-Recombinant Proteins,
pubmed-meshheading:11852778-Viral Nonstructural Proteins
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pubmed:articleTitle |
[Characterization of a panel of monoclonal antibodies to hepatitis C NS3 recombinant protein ].
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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