Source:http://linkedlifedata.com/resource/pubmed/id/11850721
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-2-18
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| pubmed:abstractText |
Biological effects of in vivo transfection of a potential anti-inflammatory gene, designated Sm16, cloned from the human parasite Schistosoma mansoni were analyzed in these studies. A single intradermal injection of a full-length cDNA of Sm16 resulted in the expression of Sm16 in the epidermis, dermis, skin migratory cells and skin-draining lymph nodes of mice for up to 7 days. Subsequently the anti-inflammatory effect of this gene expression was evaluated by inducing an inflammatory response in the skin of mice. These studies showed that Sm16 gene delivery resulted in a significant suppression of cutaneous inflammation as shown by a reduction in cutaneous edema, decrease in neutrophil infiltration, suppression of pro-inflammatory cytokine expression and down-regulation of ICAM-1 expression in the skin inflammatory site. Cells collected from the skin-draining lymph nodes showed reduced proliferation to mitogen. Multiple intradermal injection of Sm16 cDNA failed to induce any antibody response in mice for up to 8 weeks after initial injection. These findings suggest a potential for developing Sm16 gene delivery as a therapeutic agent for treating inflammatory skin disorders.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/SM16 protein, Schistosoma mansoni
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0969-7128
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
9
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
38-45
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11850721-Animals,
pubmed-meshheading:11850721-Cell Division,
pubmed-meshheading:11850721-Cytomegalovirus,
pubmed-meshheading:11850721-DNA, Helminth,
pubmed-meshheading:11850721-Dermatitis,
pubmed-meshheading:11850721-Gene Expression,
pubmed-meshheading:11850721-Gene Therapy,
pubmed-meshheading:11850721-Genetic Vectors,
pubmed-meshheading:11850721-Helminth Proteins,
pubmed-meshheading:11850721-Injections, Intradermal,
pubmed-meshheading:11850721-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11850721-Lipopolysaccharides,
pubmed-meshheading:11850721-Lymph Nodes,
pubmed-meshheading:11850721-Mice,
pubmed-meshheading:11850721-Mice, Inbred C57BL,
pubmed-meshheading:11850721-Neutrophil Infiltration,
pubmed-meshheading:11850721-Schistosoma mansoni,
pubmed-meshheading:11850721-Skin
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| pubmed:year |
2002
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| pubmed:articleTitle |
Suppression of cutaneous inflammation by intradermal gene delivery.
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| pubmed:affiliation |
Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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