Linked Life Data

11841909

Source:http://linkedlifedata.com/resource/pubmed/id/11841909

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-2-13
pubmed:abstractText
The following experiments investigated the use of sheep serum (SS) as a capacitating agent for red deer (Cervus elaphus) sperm during in vitro fertilisation. Red deer oocytes were collected at slaughter and matured in vitro for 24h in TCM-199 supplemented with 10% foetal calf serum, 10 microg ml(-1) FSH and LH, and 1microg ml(-1) of oestradiol. Fertilisation medium was IVF-SOF modified to contain 5mM Ca(2+) and no glucose. Experiment 1 investigated the addition of heparin, BSA (8 mg ml(-1)) or 20% SS. All oocytes were penetrated when IVF-SOF was supplemented with SS compared to 10 and 0% penetration when either heparin or BSA was present (P<0.01). However, 43.8% of these oocytes were polyspermic when the medium contained SS. In Experiment 2, the effect of sperm concentration on penetration rates during in vitro fertilisation was investigated. Total sperm penetration and monospermic penetration rates increased with increased sperm concentrations in a log linear manner (P<0.001) and both approached an asymptote at 0.4 x 10(6) sperm ml(-1) with 93.6 and 77% for total and monospermic penetration, respectively. Polyspermic fertilisation also increased with increasing sperm concentrations (P<0.05) but was variable (range 3.5+/-4.2 to 42.3+/-10.6%), especially at the lower sperm concentrations. Experiment 3 investigated the viability of these oocytes after transfer into red deer recipients. Fifteen 2- and 4-cell embryos were transferred into the oviducts of synchronized recipients 28 h post in vitro insemination. An additional fourteen embryos (8-10 cell) were transferred into synchronised recipients after 48 h of in vitro culture in either SOFaaBSA (n=10) or on red deer epithelial oviduct monolayers (n=4). Five (33% 5/15) of the recipients that received 2- and 4-cell embryos were pregnant at Day 45 (verified by ultrasonography) and four recipients subsequently calved. One recipient receiving an embryo cultured in SOFaaBSA was pregnant at Day 45 and subsequently calved. The birth of five normal calves indicate that full developmental competence of red deer oocytes matured and fertilised in vitro can be achieved by the techniques described.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0378-4320
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-98
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11841909-Animals, pubmed-meshheading:11841909-Blood, pubmed-meshheading:11841909-Culture Media, pubmed-meshheading:11841909-Culture Techniques, pubmed-meshheading:11841909-Deer, pubmed-meshheading:11841909-Embryo Transfer, pubmed-meshheading:11841909-Estradiol, pubmed-meshheading:11841909-Female, pubmed-meshheading:11841909-Fertilization in Vitro, pubmed-meshheading:11841909-Follicle Stimulating Hormone, pubmed-meshheading:11841909-Heparin, pubmed-meshheading:11841909-Luteinizing Hormone, pubmed-meshheading:11841909-Male, pubmed-meshheading:11841909-Pregnancy, pubmed-meshheading:11841909-Pregnancy Outcome, pubmed-meshheading:11841909-Serum Albumin, Bovine, pubmed-meshheading:11841909-Sheep, pubmed-meshheading:11841909-Sperm Capacitation, pubmed-meshheading:11841909-Sperm Count
pubmed:year
2002
pubmed:articleTitle
Development of in vitro embryo production systems for red deer (Cervus elaphus). Part 3. In vitro fertilisation using sheep serum as a capacitating agent and the subsequent birth of calves.
pubmed:affiliation
Reproductive Technologies Group, AgResearch Ruakura, Private Bag 3123, Hamilton, New Zealand. debra.berg@agresearch.co.nz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't