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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-2-8
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pubmed:abstractText |
Facilitative glucose transporters exhibit variable hexose affinity and tissue-specific expression. These characteristics contribute to specialized metabolic properties of cells. Here we describe the characterization of a novel glucose transporter-like molecule, GLUT-12. GLUT-12 was identified in MCF-7 breast cancer cells by homology to the insulin-regulatable glucose transporter GLUT-4. The GLUT-12 cDNA encodes 617 amino acids, which possess features essential for sugar transport. Di-leucine motifs are present in NH(2) and COOH termini at positions similar to the GLUT-4 FQQI and LL targeting motifs. GLUT-12 exhibits 29% amino acid identity with GLUT-4 and 40% to the recently described GLUT-10. Like GLUT-10, a large extracellular domain is predicted between transmembrane domains 9 and 10. Genomic organization of GLUT-12 is highly conserved with GLUT-10 but distinct from GLUTs 1-5. Immunofluorescence showed that, in the absence of insulin, GLUT-12 is localized to the perinuclear region in MCF-7 cells. Immunoblotting demonstrated GLUT-12 expression in skeletal muscle, adipose tissue, and small intestine. Thus GLUT-12 is potentially part of a second insulin-responsive glucose transport system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transport Proteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SLC2A4 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0193-1849
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E733-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11832379-Amino Acid Sequence,
pubmed-meshheading:11832379-Blotting, Northern,
pubmed-meshheading:11832379-Brain Chemistry,
pubmed-meshheading:11832379-Breast Neoplasms,
pubmed-meshheading:11832379-Cell Membrane,
pubmed-meshheading:11832379-Cloning, Molecular,
pubmed-meshheading:11832379-DNA, Complementary,
pubmed-meshheading:11832379-Embryo, Mammalian,
pubmed-meshheading:11832379-Exons,
pubmed-meshheading:11832379-Female,
pubmed-meshheading:11832379-Fluorescent Antibody Technique,
pubmed-meshheading:11832379-Gene Library,
pubmed-meshheading:11832379-Glucose Transport Proteins, Facilitative,
pubmed-meshheading:11832379-Glucose Transporter Type 4,
pubmed-meshheading:11832379-Humans,
pubmed-meshheading:11832379-Immunoblotting,
pubmed-meshheading:11832379-Immunohistochemistry,
pubmed-meshheading:11832379-Infant, Newborn,
pubmed-meshheading:11832379-Insulin,
pubmed-meshheading:11832379-Introns,
pubmed-meshheading:11832379-Molecular Sequence Data,
pubmed-meshheading:11832379-Monosaccharide Transport Proteins,
pubmed-meshheading:11832379-Muscle Proteins,
pubmed-meshheading:11832379-Organ Specificity,
pubmed-meshheading:11832379-Pregnancy,
pubmed-meshheading:11832379-RNA, Messenger,
pubmed-meshheading:11832379-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11832379-Sequence Homology,
pubmed-meshheading:11832379-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Identification of a novel glucose transporter-like protein-GLUT-12.
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pubmed:affiliation |
Department of Medicine, The University of Melbourne, St. Vincent's Hospital Melbourne, Fitzroy, Victoria 3065, Australia 4072.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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