Linked Life Data

11826113

Source:http://linkedlifedata.com/resource/pubmed/id/11826113

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-2-4
pubmed:abstractText
The transcription factor Engrailed (En) directs, in the cockroach cercal system, the shape of the axonal arborization and the choice of postsynaptic partners of an identified sensory neuron (6m). Knock-out of En using double-stranded RNA interference transforms 6m so that it resembles a neighboring neuron that normally does not express the en gene, has a different arbor anatomy, and makes different connections. We characterized the development of 6m and perturbed en expression at different stages. Our results show that En is not required before birth for 6m to become a neuron, but that it is required in the postmitotic neuron to control axonal arborization and synaptic specificity. Knock-out of En after 6m has entered the CNS does not change the axonal trajectory and has minor effects on axonal branches but causes the formation of synaptic connections typical of an En-negative cell. This suggests that En controls target recognition molecules independently from those guiding the axon. In contrast, double-stranded RNA injection 1 d later does not have any effects on the phenotype of 6m, suggesting that the period of synapse formation is over by the time En levels have fallen or, if synapse turnover occurs, that En is not required to maintain the specificity of synaptic connections. We conclude that persistent en expression is required to determine successive stages in the differentiation of the neuron, suggesting that it is not far upstream from those genes encoding axon guidance and synaptic recognition molecules.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
832-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Persistent engrailed expression is required to determine sensory axon trajectory, branching, and target choice.
pubmed:affiliation
Institute of Neurobiology and Department of Physiology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico 00901.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.