Linked Life Data

11818575

Source:http://linkedlifedata.com/resource/pubmed/id/11818575

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-2-6
pubmed:abstractText
We introduce coherent infrared emission interferometry as a chi(2) vibrational spectroscopy technique and apply it to studying the initial dynamics upon photoactivation of myoglobin (Mb). By impulsive excitation (using 11-fs pulses) of a Mb crystal, vibrations that couple to the optical excitation are set in motion coherently. Because of the order in the crystal lattice the coherent oscillations of the different proteins in the crystal that are associated with charge motions give rise to a macroscopic burst of directional multi-teraHertz radiation. This radiation can be detected in a phase-sensitive way by heterodyning with a broad-band reference field. In this way both amplitude and phase of the different vibrations can be obtained. We detected radiation in the 1,000-1,500 cm(-1) frequency region, which contains modes sensitive to the structure of the heme macrocycle, as well as peripheral protein modes. Both in carbonmonoxy-Mb and aquomet-Mb we observed emission from six modes, which were assigned to heme vibrations. The phase factors of the modes contributing to the protein electric field show a remarkable consistency, taking on values that indicate that the dipoles are created "emitting" at t = 0, as one would expect for impulsively activated modes. The few deviations from this behavior in Mb-CO we propose are the result of these modes being sensitive to the photodissociation process and severely disrupted by it.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-10490029, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-10512835, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-10706294, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-1191643, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-1332761, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-3415972, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-7035792, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-7225362, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-7552707, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-7756319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-8939867, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-9506944, http://linkedlifedata.com/resource/pubmed/commentcorrection/11818575-9860966
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1323-8
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Coherent infrared emission from myoglobin crystals: an electric field measurement.
pubmed:affiliation
Laboratory for Optical Biosciences, INSERM U451, Centre National de la Recherche Scientifique Unité Mixte de Recherche 7645, Ecole Polytechnique-Ecole Nationale Supárievre de Techniques Avancáes ENSTA, 91128 Palaiseau Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't