Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-1-30
pubmed:abstractText
The heterodimeric hypoxia-inducible factor (HIF)-1 is a transcriptional master regulator of several genes involved in mammalian oxygen homeostasis, including erythropoietin, vascular endothelial growth factor, and factors involved in glucose transport and metabolism. The mouse Hif1a gene is expressed from two distinct promoter/first exon combinations resulting in tissue-specific (mHIF-1alphaI.1) and ubiquitous (mHIF-1alphaI.2) mRNA isoforms. By in situ hybridization, we detected mHIF-1alphaI.1 mRNA exclusively in the elongated spermatids of the testis. In vitro studies indicated that the switch from mHIF-1alphaI.2 to mHIF-1alphaI.1 mRNA expression does not occur at the premeiotic stages of mouse spermatogenesis. Exposure of mice to hypoxic conditions induced mHIF-1alphaI.2 protein in spermatocytes and probably in Sertoli cells but not in spermatogonia. In contrast, expression of the putative mHIF-1alphaI.1 protein in spermatozoa of the testis and epididymis was oxygen independent and located to the midpiece of the spermatozoal flagellum. Both the switch in transcript expression during spermiogenesis and the unexpected protein localization in mature sperm cells suggest a so far unrecognized function of HIF-1alpha.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
234-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11818497-Animals, pubmed-meshheading:11818497-Anoxia, pubmed-meshheading:11818497-Cell Line, pubmed-meshheading:11818497-DNA-Binding Proteins, pubmed-meshheading:11818497-Gene Expression Regulation, Developmental, pubmed-meshheading:11818497-Hypoxia-Inducible Factor 1, pubmed-meshheading:11818497-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:11818497-Immunohistochemistry, pubmed-meshheading:11818497-In Situ Hybridization, pubmed-meshheading:11818497-Leydig Cells, pubmed-meshheading:11818497-Male, pubmed-meshheading:11818497-Meiosis, pubmed-meshheading:11818497-Mice, pubmed-meshheading:11818497-Nuclear Proteins, pubmed-meshheading:11818497-Organ Specificity, pubmed-meshheading:11818497-Oxygen, pubmed-meshheading:11818497-Protein Isoforms, pubmed-meshheading:11818497-Protein Transport, pubmed-meshheading:11818497-RNA, Messenger, pubmed-meshheading:11818497-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11818497-Sertoli Cells, pubmed-meshheading:11818497-Sperm Tail, pubmed-meshheading:11818497-Spermatids, pubmed-meshheading:11818497-Spermatogenesis, pubmed-meshheading:11818497-Testis, pubmed-meshheading:11818497-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
Isoform-specific expression of hypoxia-inducible factor-1alpha during the late stages of mouse spermiogenesis.
pubmed:affiliation
Max-Planck-Institute for Physiological and Clinical Research, Department of Molecular Cell Biology, D-61231 Bad Nauheim, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't