Source:http://linkedlifedata.com/resource/pubmed/id/11806978
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2002-1-24
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| pubmed:abstractText |
Stromal-derived factor 1 (SDF-1) is a -CXC- chemokine that plays a critical role in embryonic and adult hematopoiesis, and its specific receptor, CXCR4, has been implicated in stem cell homing. In this study, it is shown that the addition of SDF-1 to long-term cultures (LTCs) of normal human marrow can selectively, reversibly, and specifically block the S-phase entry of primitive quiescent erythroid and granulopoietic colony-forming cells (CFCs) present in the adherent layer. Conversely, addition of anti-SDF-1 antibody or SDF-1(G2), a specific CXCR4 antagonist, to preactivated human LTCs prevented both types of primitive CFCs from re-entering a quiescent state, demonstrating that endogenous SDF-1 contributes to the control of primitive CFC proliferation in the LTC system. Interestingly, SDF-1 failed to arrest the proliferation of primitive chronic myeloid leukemia CFCs in the adherent layer of LTCs containing normal marrow stromal cells. In vivo, injection of SDF-1 arrested the cycling of normal human LTC-initiating cells as well as primitive CFCs in the marrow of nonobese diabetic/severe combined immunodeficient mice engrafted with human cord blood cells. Conversely, injection of the antagonist, SDF-1(G2), reactivated the cycling of quiescent primitive human CFCs present in the marrow of mice engrafted with human marrow cells. These studies are the first to demonstrate a potential physiological role of SDF-1 in regulating the cell-cycle status of primitive hematopoietic cells and suggest that the deregulated cycling activity of primitive chronic myeloid leukemia (CML) cells is due to the BCR-ABL-mediated disruption of a pathway shared by multiple chemokine receptors.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
99
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
792-9
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11806978-Animals,
pubmed-meshheading:11806978-Bone Marrow Cells,
pubmed-meshheading:11806978-Bone Marrow Transplantation,
pubmed-meshheading:11806978-Cell Cycle,
pubmed-meshheading:11806978-Cell Division,
pubmed-meshheading:11806978-Cells, Cultured,
pubmed-meshheading:11806978-Chemokine CXCL12,
pubmed-meshheading:11806978-Chemokines, CXC,
pubmed-meshheading:11806978-Fetal Blood,
pubmed-meshheading:11806978-Graft Survival,
pubmed-meshheading:11806978-Hematopoietic Stem Cells,
pubmed-meshheading:11806978-Humans,
pubmed-meshheading:11806978-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:11806978-Mice,
pubmed-meshheading:11806978-Mice, Inbred NOD,
pubmed-meshheading:11806978-Mice, SCID,
pubmed-meshheading:11806978-Stem Cells,
pubmed-meshheading:11806978-Transplantation, Heterologous
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
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| pubmed:affiliation |
Terry Fox Laboratory, British Columbia Cancer Agency, 601 West 10th Ave, Vancouver, BC, V5Z 1L3, Canada.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|