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pubmed:abstractText |
The availability of combinatorial T cell epitope libraries made using a phage display system would be useful for identifying the antigens recognized by T cells of unknown specificity. To this end, we have investigated here whether single chain-MHC class I molecules (scMHC-I) could be expressed together with antigenic peptides on filamentous phages. The results show that filamentous phages can express scMHC-I. Moreover, the expressed scMHC-I was able to bind antigenic peptide. These data support the use of combinatorial scMHC/T cell epitope libraries for screening potential T cell antigens.
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pubmed:affiliation |
Rheumatology, Immunology, and Genetic Program, Institute of Medical Science, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8512, Japan.
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