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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2002-3-18
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pubmed:abstractText |
Degradation of platelet-activating factor (PAF) was examined by incubating PAF with macrophages from PAF receptor-deficient mice. The degradation rate was halved as compared with wild-type mice. The reduction of the rate was comparable with the presence of a PAF antagonist WEB 2086 in wild-type cells. PAF was internalized rapidly (t(12) approximately 1 min) into wild-type macrophages. The PAF internalization was inhibited by the treatment of 0.45 m sucrose but was not affected by phorbol 12-myristate 13-acetate, suggesting that PAF internalizes into macrophages with its receptor in a clathrin-dependent manner. Internalized PAF was degraded into lyso-PAF with a half-life of 20 min. Treatment of concanavalin A inhibited the conversion of PAF into lyso-PAF, suggesting that uptake of PAF enhances PAF degradation. Lyso-PAF was subsequently metabolized into 1-alkyl-2-acyl-phosphatidylcholine. In addition, release of PAF acetylhydrolase from macrophages was enhanced when wild-type macrophages were stimulated with PAF but not from macrophages of PAF receptor-deficient mice. Thus, the PAF stimulation of macrophages leads to its degradation through both intracellular and extracellular mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Alkyl-2-acetylglycerophosphocholin...,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrose,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/platelet activating factor receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9722-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11786559-1-Alkyl-2-acetylglycerophosphocholine Esterase,
pubmed-meshheading:11786559-Animals,
pubmed-meshheading:11786559-Blotting, Western,
pubmed-meshheading:11786559-Chromatography, Thin Layer,
pubmed-meshheading:11786559-Concanavalin A,
pubmed-meshheading:11786559-Dose-Response Relationship, Drug,
pubmed-meshheading:11786559-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11786559-Genotype,
pubmed-meshheading:11786559-Ligands,
pubmed-meshheading:11786559-Lipid Metabolism,
pubmed-meshheading:11786559-Macrophages,
pubmed-meshheading:11786559-Mice,
pubmed-meshheading:11786559-Mice, Inbred C57BL,
pubmed-meshheading:11786559-Mice, Knockout,
pubmed-meshheading:11786559-Phosphatidylcholines,
pubmed-meshheading:11786559-Phospholipases A,
pubmed-meshheading:11786559-Platelet Activating Factor,
pubmed-meshheading:11786559-Platelet Membrane Glycoproteins,
pubmed-meshheading:11786559-Protein Binding,
pubmed-meshheading:11786559-Receptors, Cell Surface,
pubmed-meshheading:11786559-Receptors, G-Protein-Coupled,
pubmed-meshheading:11786559-Sucrose,
pubmed-meshheading:11786559-Tetradecanoylphorbol Acetate,
pubmed-meshheading:11786559-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Receptor-dependent metabolism of platelet-activating factor in murine macrophages.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113-0033 Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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