|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
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|
pubmed:dateCreated |
2002-1-10
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|
pubmed:abstractText |
Hybridomas producing monoclonal antibodies (MAb) to Marburg virus glycoprotein were prepared by splicing of mouse myeloma cells (NSO and X.63-Ag6.653 strains) and splenocytes of immunized BALB/c mice. Cultural and MAb-producing properties of hybridomas were studied. The production of MAb during serial passages 15-30 was confirmed. MAb titers in culture fluids were 1:4096-1:8152, in immune ascitic fluids of BALB/c mice 1:409,600-1:638,400. The possibility of using MAbs as a component of ELISA test system was demonstrated. The sensitivity of ELISA-MAb method for Marburg virus was 1 x 10(3) PFU/mu.
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pubmed:language |
rus
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:issn |
0507-4088
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:volume |
46
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
21-4
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|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11785382-Animals,
pubmed-meshheading:11785382-Antibodies, Monoclonal,
pubmed-meshheading:11785382-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11785382-Hybridomas,
pubmed-meshheading:11785382-Marburgvirus,
pubmed-meshheading:11785382-Mice,
pubmed-meshheading:11785382-Mice, Inbred BALB C,
pubmed-meshheading:11785382-Serial Passage,
pubmed-meshheading:11785382-Viral Envelope Proteins,
pubmed-meshheading:11785382-Viral Structural Proteins
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|
pubmed:articleTitle |
[Production and study of hybridomas, producing monoclonal antibodies to the structural glycoprotein of Marburg virus].
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pubmed:publicationType |
Journal Article,
English Abstract
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