Linked Life Data

11773099

Source:http://linkedlifedata.com/resource/pubmed/id/11773099

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-1-4
pubmed:abstractText
A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.). The analytical sensitivity is less than one organism per reaction. Results for Bordetella culture and/or direct fluorescent antibody testing and a second LightCycler PCR assay (target, pertussis toxin gene) were compared to results of the LC-PCR-IS assay for 111 nasopharyngeal swabs submitted for pertussis testing. Of the specimens, 12 were positive (9 B. pertussis and 3 B. parapertussis) and 68 specimens were negative by all methods. Three other specimens were positive for B. pertussis by at least two of the methods (including the LC-PCR-IS assay), and another 28 specimens were positive for B. pertussis by the LC-PCR-IS assay only. No specimens were negative by the LC-PCR-IS assay and positive by the other methods. A conventional PCR method (target, IS481) was also compared to the LC-PCR-IS assay for a different group of nasopharyngeal swab specimens (n = 96): 44 specimens were positive and 41 specimens were negative for B. pertussis with both PCR methods. Nine specimens were positive for B. pertussis by the LC-PCR-IS assay and negative by the conventional PCR assay, and two specimens were positive for B. pertussis by the conventional PCR assay and negative by the LC-PCR-IS assay. Positivity of the two assays was not significantly different (P = 0.0654). The insertion sequence IS481 is also present in Bordetella holmesii; specimens containing B. holmesii may yield false-positive results. The LC-PCR-IS assay takes approximately 45 min to complete post-nucleic acid extraction, compared to 24 h for the conventional PCR assay previously used in our laboratory. The LC-PCR-IS assay is easier to perform than the conventional PCR assay, and the closed system decreases the chance of contamination. All of these characteristics represent a significant improvement in the detection of B. pertussis and B. parapertussis in nasopharyngeal specimens.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-10341183, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-10447026, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-10449467, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-10834997, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-2229381, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-2560238, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-7699023, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-8027309, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-8195394, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-8370741, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-8429539, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-8606353, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-9316885, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-9350776, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-9502460, http://linkedlifedata.com/resource/pubmed/commentcorrection/11773099-9986820
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
96-100
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens.
pubmed:affiliation
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Evaluation Studies