Linked Life Data

11772823

Source:http://linkedlifedata.com/resource/pubmed/id/11772823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-1-4
pubmed:abstractText
Volatile anesthetics and K(+) channel openers inhibit spontaneous contractions in myometrial smooth muscle. Volatile anesthetics modulate K(+) channel activity. We investigated the role of two K(+) channel blockers on the effect of sevoflurane in pregnant rat myometrium. Term pregnant rat uteri were excised, and cross-sectional myometrial strips were mounted for isometric force recording. Sevoflurane inhibited the amplitude and frequency of spontaneous myometrial contractions in a concentration-dependent manner. The maximal inhibition measured in amplitude and frequency of spontaneous myometrial contractions with sevoflurane (at 3 minimum alveolar anesthetic concentration) was 44.32% and 33.32% of control contractions, respectively. Tetraethylammonium (TEA) and glibenclamide, K(+) channel blockers, increased spontaneous myometrial contractions in a concentration-dependent manner. Sevoflurane responses were repeated at concentrations with no effect on spontaneous contractility of TEA, a Ca(2+)-activated K(+) channel blocker, and glibenclamide, an adenosine triphosphate-sensitive K(+) channel blocker, in myometrial strips. TEA (3.10(-4) M) caused a significant reduction in sevoflurane-induced inhibitor responses, but glibenclamide (10(-6) M) did not. Sevoflurane-induced maximal inhibition (at 3 minimum alveolar anesthetic concentration) on amplitude and frequency of spontaneous myometrial contractions in the presence of TEA (3.10(-4) M) was 31.85% and 22.33% of control contractions, respectively (P < 0.05). These results suggest that the in vitroapplication of sevoflurane inhibited the amplitude and frequency of spontaneous myometrial contractions in pregnant rats in a concentration-dependent manner. Such inhibition was reduced by TEA. The inhibition of myometrial smooth muscle induced by sevoflurane seems to be mediated, at least in part, via activation of Ca(2+)-activated K(+) channels, because inhibition was reduced by TEA. IMPLICATIONS: In this study, we found that sevoflurane causes significantly decreased myometrial contractile activity in pregnant rats. The inhibition of myometrial smooth muscle induced by sevoflurane seems to be mediated, at least in part, via activation of Ca(2+)-activated K(+) channels, because inhibition was reduced by tetraethylammonium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0003-2999
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
174-8, table of contents
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11772823-Adenosine Triphosphate, pubmed-meshheading:11772823-Anesthetics, Inhalation, pubmed-meshheading:11772823-Animals, pubmed-meshheading:11772823-Dose-Response Relationship, Drug, pubmed-meshheading:11772823-Female, pubmed-meshheading:11772823-Glyburide, pubmed-meshheading:11772823-Methyl Ethers, pubmed-meshheading:11772823-Muscle Contraction, pubmed-meshheading:11772823-Muscle Relaxation, pubmed-meshheading:11772823-Myometrium, pubmed-meshheading:11772823-Potassium Channel Blockers, pubmed-meshheading:11772823-Potassium Channels, pubmed-meshheading:11772823-Potassium Channels, Calcium-Activated, pubmed-meshheading:11772823-Pregnancy, pubmed-meshheading:11772823-Pregnancy, Animal, pubmed-meshheading:11772823-Rats, pubmed-meshheading:11772823-Rats, Wistar, pubmed-meshheading:11772823-Tetraethylammonium
pubmed:year
2002
pubmed:articleTitle
The role of K(+) channels on the inhibitor effect of sevoflurane in pregnant rat myometrium.
pubmed:affiliation
Department of Anesthesiology, Cumhuriyet University School of Medicine, Sivas, Turkey. haluk.kafali@isnet.net.tr
pubmed:publicationType
Journal Article, In Vitro