Linked Life Data

11755115

Source:http://linkedlifedata.com/resource/pubmed/id/11755115

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-12-28
pubmed:abstractText
In the present study we have employed fura-2 loaded isolated mouse pancreatic acinar cells to monitor the effect that xanthine oxidase (XOD)-catalyzed reactive oxygen species generation presents on Ca(2+) mobilization by the secretagogue cholecystokinin octapeptide (CCK-8). Our results show that perfusion of pancreatic acinar cells with CCK-8 at a physiological concentration (20 pM) induced low frequency oscillations in intracellular free calcium concentration ([Ca(2+)](i)) at a rate of 1 per minute; this oscillatory pattern was completely inhibited by the introduction in the perifusion medium of 20 mU/mL XOD to generate reactive oxygen species. In addition, perfusion of pancreatic acinar cells with 20 mU/mL XOD in the absence of extracellular calcium led to a transient increase in [Ca(2+)](i,) that blocked the initiation of the Ca(2+) signals in response to 20 pM CCK-8. Similarly, XOD was also able to block acetylcholine evoked Ca(2+) spikes. However, reactive oxygen species had no effect either on Ca(2+) extrusion or on re-uptake into intracellular stores, but CCK-8-evoked Ca(2+) entry was reduced by XOD. In conclusion, our results show that XOD-evoked reactive oxygen species generation leads to a reduction either of Ca(2+) mobilization, following stimulation of pancreatic acinar cells with the Ca(2+)-mobilizing agonists CCK-8 and acetylcholine, and Ca(2+) influx evoked by CCK-8 depletion of intracellular stores. The possible XOD inhibitory mechanism on Ca(2+) mobilization by agonists is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1621-7
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Effect of xanthine oxidase-catalyzed reactive oxygen species generation on secretagogue-evoked calcium mobilization in mouse pancreatic acinar cells.
pubmed:affiliation
Department of Physiology, University of Extremadura, Faculty of Veterinary Sciences, P.O. Box 643, 10071, Cáceres, Spain. agmateos@unex.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't