Linked Life Data

11753682

Source:http://linkedlifedata.com/resource/pubmed/id/11753682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
55
pubmed:dateCreated
2001-12-25
pubmed:abstractText
IEX-1, an immediate early gene, is widely expressed in epithelial and endothelial tissues, and is altered by a variety of growth regulatory factors. We have shown that expression of IEX-1 in keratinocytes increases the growth rate of these cells. The effects of IEX-1 on apoptosis, however, are unclear. To clarify the effects of IEX-1 on apoptosis, we investigated the effects of IEX-1 expression in keratinocytes (HaCaT cells) in the basal state and after the induction of cellular stress. Under normal, non-stressed conditions, both control (HaCaT) and IEX-1-transfected (IEX-HaCaT) cell lines showed no significant differences in the activity of a key apoptotic enzyme, caspase 3 despite significantly higher levels of IEX-1 expression. IEX-HaCaT cells grew faster than HaCaT cells. When both cell lines were irradiated with ultraviolet B radiation, caspase 3 activity increased to a greater extent in the IEX-HaCaT cells than in HaCaT cells. Camptothecin increased caspase 3 activity twice as much in the IEX-HaCaT cells when compared to HaCaT cells. When histone-complex DNA fragments were measured in IEX-HaCaT or HaCaT cells following UVB irradiation or treatment with camptothecin, significantly higher amounts of nucleosomes were seen in the IEX-HaCaT transfected cells. Likewise, serum deprivation induced higher degrees of apoptosis in IEX-HaCaT cells than in HaCaT cells. We conclude that overexpression of IEX-1 in HaCaT keratinocytes increases the growth rate of cells under basal conditions; in the basal state the rate of apoptosis is unchanged. However, the rate of apoptosis increases in IEX-1 overexpressing HaCaT keratinocytes after cells are subjected to stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7992-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11753682-Apoptosis, pubmed-meshheading:11753682-Apoptosis Regulatory Proteins, pubmed-meshheading:11753682-Camptothecin, pubmed-meshheading:11753682-Caspase 3, pubmed-meshheading:11753682-Caspases, pubmed-meshheading:11753682-Cell Adhesion, pubmed-meshheading:11753682-Cell Division, pubmed-meshheading:11753682-Enzyme Activation, pubmed-meshheading:11753682-Genes, Immediate-Early, pubmed-meshheading:11753682-Humans, pubmed-meshheading:11753682-Immediate-Early Proteins, pubmed-meshheading:11753682-Keratinocytes, pubmed-meshheading:11753682-Membrane Glycoproteins, pubmed-meshheading:11753682-Membrane Proteins, pubmed-meshheading:11753682-Neoplasm Proteins, pubmed-meshheading:11753682-Protein Transport, pubmed-meshheading:11753682-RNA, Messenger, pubmed-meshheading:11753682-Transfection, pubmed-meshheading:11753682-Tumor Cells, Cultured, pubmed-meshheading:11753682-Ultraviolet Rays
pubmed:year
2001
pubmed:articleTitle
IEX-1, an immediate early gene, increases the rate of apoptosis in keratinocytes.
pubmed:affiliation
Department of Medicine, Mayo Clinic and Foundation, Rochester, Minnesota, MN 55905, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't