Linked Life Data

11750066

Source:http://linkedlifedata.com/resource/pubmed/id/11750066

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-12-25
pubmed:databankReference
pubmed:abstractText
The Suppression Subtractive Hybridization (SSH) was used to isolate large numbers of low temperature-induced genes from the cold-treated winter barley (Hordeum vulgare L. cv. Dongbori). One hundred and sixty blti (barley low temperature-induced) cDNA clones were obtained. Northern blot analyses showed that several blti clones were differentially expressed by treatment of low temperature, NaCl, dehydration and ABA. One of the clones, blti2, was induced from 3 to 72 h after cold treatment while its transcript was detected only at 12 h after ABA treatment, indicating that the expression of blti2 by low temperature was regulated by an ABA-independent pathway. The full-length cDNA sequences were 944 nucleotides long and the open reading frame consisted of 492 nucleotides encoding 164 amino acids. Nucleotide sequences showed no sequence homology with the previously reported low temperature-responsive (LTR) barley genes, and the deduced amino acid sequences revealed that the blti2 contains three membrane-spanning regions. These results suggest that blti2 is a novel transmembrane protein induced by low temperature.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
1522
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeum vulgare L.).
pubmed:affiliation
Graduate School of Biotechnology, Korea University, Seoul, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't