Source:http://linkedlifedata.com/resource/pubmed/id/11747608
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2001-12-18
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pubmed:abstractText |
Heterogeneous nuclear ribonucleoprotein K (hnRNP K), a component of hnRNP particles, is involved in several steps of gene expression regulation. Dengue (DEN) virus, a member of the Flaviviridae, is the primary cause of illnesses such as dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. In mature DEN virus particles, the core protein is a structural protein that forms a nucleocapsid complex with genomic RNA. Very little of its biologic functions is known. Here, using an in vitro binding assay and coimmunoprecipitation analysis, we report a protein-protein interaction between the DEN virus core protein and hnRNP K. The C-terminal hydrophilic region of the DEN virus core protein, spanning amino acid residues 73 to 100, is required for such interaction. Results of glutathione-S transferase binding assays indicated that the core protein-hnRNP K interaction might be abolished in the presence of hnRNP K cognate nucleic acids. Furthermore, in a cotransfection experiment, the repressive effect of hnRNP K on C/EBPbeta-mediated transcription activation could be reversed by full-length DEN virus core protein but not by a truncated form containing amino acids 1-72. Our results suggest that, on DEN virus infection, the multiple functions of cellular hnRNP K may be affected by the virus core protein.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1044-5498
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
569-77
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11747608-Amino Acid Sequence,
pubmed-meshheading:11747608-Animals,
pubmed-meshheading:11747608-Cell Line,
pubmed-meshheading:11747608-Dengue,
pubmed-meshheading:11747608-Dengue Virus,
pubmed-meshheading:11747608-Heterogeneous-Nuclear Ribonucleoprotein K,
pubmed-meshheading:11747608-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:11747608-Molecular Sequence Data,
pubmed-meshheading:11747608-RNA,
pubmed-meshheading:11747608-RNA-Binding Proteins,
pubmed-meshheading:11747608-Ribonucleoproteins,
pubmed-meshheading:11747608-Viral Core Proteins,
pubmed-meshheading:11747608-Virus Replication
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pubmed:year |
2001
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pubmed:articleTitle |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein.
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pubmed:affiliation |
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. chingjin@gate.sinica.edu.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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