Linked Life Data

11743719

Source:http://linkedlifedata.com/resource/pubmed/id/11743719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-12-17
pubmed:abstractText
The daf-2 insulin-like receptor pathway regulates development and life-span in Caenorhabditis elegans. Reduced DAF-2 signaling leads to changes in downstream targets via the daf-16 gene, a fork-head transcription factor which is regulated by DAF-2, and results in extended life-span. Here, we describe the first identification of genes whose expression is controlled by the DAF-2 signaling cascade. dao-1, dao-2, dao-3, dao-4, dao-8 and dao-9 are down-regulated in daf-2 mutant adults compared to wild-type adults, whereas dao-5, dao-6 and dao-7 are up-regulated. The latter genes are negatively regulated by DAF-2 signaling and positively regulated by DAF-16. Positive regulation by DAF-2 on dao-1, dao-4 and dao-8 was mediated by DAF-16, whereas daf-16 mediates only part of DAF-2 signaling for dao-2 and dao-9. Regulation by DAF-2 is most likely DAF-16 independent for dao-3 and hsp-90. RNA levels of dao-5 and dao-6 showed elevated expression in daf-2 adults, as well as being strongly expressed in dauer larvae. In contrast, hsp-90 transcript levels are low in daf-2 mutant adults though they are enriched in dauer larvae, indicating overlapping but not identical mechanisms of efficient life maintenance in stress-resistant dauer larvae and long-lived daf-2 mutant adults. dao-1, dao-8 and dao-9 are homologs of the FK506 binding proteins that interact with the mammalian insulin pathway. dao-3 encodes a putative methylenetetrahydrofolate dehydrogenase. DAO-5 shows 33 % identity with human nucleolar phosphoprotein P130. dao-7 is similar to the mammalian ZFP36 protein. Distinct regulatory patterns of dao genes implicate their diverse positions within the signaling network of DAF-2 pathway, and suggest they have unique contributions to development, metabolism and longevity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-2836
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
314
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1017-28
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11743719-Aging, pubmed-meshheading:11743719-Amino Acid Sequence, pubmed-meshheading:11743719-Animals, pubmed-meshheading:11743719-Base Sequence, pubmed-meshheading:11743719-Caenorhabditis elegans, pubmed-meshheading:11743719-Caenorhabditis elegans Proteins, pubmed-meshheading:11743719-Cloning, Molecular, pubmed-meshheading:11743719-Gene Expression Profiling, pubmed-meshheading:11743719-Gene Expression Regulation, pubmed-meshheading:11743719-Genes, Helminth, pubmed-meshheading:11743719-Larva, pubmed-meshheading:11743719-Molecular Sequence Data, pubmed-meshheading:11743719-Multigene Family, pubmed-meshheading:11743719-Mutation, pubmed-meshheading:11743719-RNA, Double-Stranded, pubmed-meshheading:11743719-RNA, Messenger, pubmed-meshheading:11743719-Receptor, Insulin, pubmed-meshheading:11743719-Reproducibility of Results, pubmed-meshheading:11743719-Signal Transduction, pubmed-meshheading:11743719-Tacrolimus Binding Proteins, pubmed-meshheading:11743719-Transcription Factors
pubmed:year
2001
pubmed:articleTitle
DAF-16-dependent and independent expression targets of DAF-2 insulin receptor-like pathway in Caenorhabditis elegans include FKBPs.
pubmed:affiliation
Molecular Biology Program and Division of Biogerontology, University of Southern California, Los Angeles, CA 90089, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't