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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 22
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pubmed:dateCreated |
2001-12-12
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pubmed:abstractText |
FENS-1 and DFCP1 are recently discovered proteins containing one or two FYVE-domains respectively. We show that the FYVE domains in these proteins can bind PtdIns3P in vitro with high specificity over other phosphoinositides. Exogenously expressed FENS-1 localises to early endosomes: this localisation requires an intact FYVE domain and is sensitive to wortmannin inhibition. The isolated FYVE domain of FENS-1 also localises to endosomes. These results are consistent with current models of FYVE-domain function in this cellular compartment. By contrast, exogenously expressed DFCP1 displays a predominantly Golgi, endoplasmic reticulum (ER) and vesicular distribution with little or no overlap with FENS-1 or other endosomal markers. Overexpression of DFCP1 was found to cause dispersal of the Golgi compartment defined by giantin and gpp130-staining. Disruption of the FYVE domains of DFCP1 causes a shift to more condensed and compact Golgi structures and overexpression of this mutant was found to confer significant protection to the Golgi against brefeldin-induced dispersal. These properties of DFCP1 are surprising, and suggest FYVE domain-localisation and function may not be exclusively endosomal. Movies available on-line
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ZFYVE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/macrogolgin,
http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylinositol 3-phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9533
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pubmed:author |
pubmed-author:AndersonK EKE,
pubmed-author:BootmanMM,
pubmed-author:CoadwellJJ,
pubmed-author:CooperM AMA,
pubmed-author:DavidsonKK,
pubmed-author:EllsonC DCD,
pubmed-author:Erdjument-BromageHH,
pubmed-author:HawkinsP TPT,
pubmed-author:HolmesA BAB,
pubmed-author:KtistakisNN,
pubmed-author:LinZ XZX,
pubmed-author:LippPP,
pubmed-author:ManifavaMM,
pubmed-author:NazarianAA,
pubmed-author:RidleyS HSH,
pubmed-author:StephensL RLR,
pubmed-author:TempstPP,
pubmed-author:ThuringJ WJW
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3991-4000
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11739631-Androstadienes,
pubmed-meshheading:11739631-Animals,
pubmed-meshheading:11739631-Brefeldin A,
pubmed-meshheading:11739631-COS Cells,
pubmed-meshheading:11739631-Carrier Proteins,
pubmed-meshheading:11739631-Endosomes,
pubmed-meshheading:11739631-Enzyme Inhibitors,
pubmed-meshheading:11739631-Golgi Apparatus,
pubmed-meshheading:11739631-Green Fluorescent Proteins,
pubmed-meshheading:11739631-Humans,
pubmed-meshheading:11739631-Indicators and Reagents,
pubmed-meshheading:11739631-Luminescent Proteins,
pubmed-meshheading:11739631-Membrane Lipids,
pubmed-meshheading:11739631-Membrane Proteins,
pubmed-meshheading:11739631-Phosphatidylinositol Phosphates,
pubmed-meshheading:11739631-Protein Binding,
pubmed-meshheading:11739631-Protein Structure, Tertiary,
pubmed-meshheading:11739631-Protein Synthesis Inhibitors,
pubmed-meshheading:11739631-Protein Transport,
pubmed-meshheading:11739631-Proteins,
pubmed-meshheading:11739631-Recombinant Fusion Proteins,
pubmed-meshheading:11739631-Surface Plasmon Resonance,
pubmed-meshheading:11739631-Zinc Fingers
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pubmed:year |
2001
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pubmed:articleTitle |
FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments.
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pubmed:affiliation |
Inositide Laboratory, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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