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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-2-4
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pubmed:abstractText |
The transcription factor B-Myb is a cell-cycle regulated phosphoprotein involved in cell cycle progression through the transcriptional regulation of many genes. In this study, we show that the promoter of the fibroblast growth factor-4 (FGF-4) gene is strongly activated by B-Myb in HeLa cells and it can serve as a novel diagnostic tool for assessing B-Myb activity. Specifically, B-Myb deletion mutants were examined and domains of B-Myb required for activation of the FGF-4 promoter were identified. Using phosphorylation-deficient mutant forms of B-Myb, we also show that phosphorylation is essential for B-Myb activity. Moreover, a mutant form of B-Myb, which lacks all identified phosphorylation sites and which has little activity, can function as a dominant-negative and suppress wild-type B-Myb activity. Acetylation is another post-translational modification known to affect the activity of other Myb family members. We show that B-Myb is acetylated by the co-activator p300. We also show that the bromo and histone acetyltransferase domains of p300 are sufficient to interact with and acetylate B-Myb. These data indicate that phosphorylation of B-Myb is an essential modification for activity and that acetylation of B-Myb may play a role in B-Myb activity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FGF4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/MYBL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4088-97
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11733503-Acetylation,
pubmed-meshheading:11733503-Animals,
pubmed-meshheading:11733503-Base Sequence,
pubmed-meshheading:11733503-CHO Cells,
pubmed-meshheading:11733503-Cell Cycle Proteins,
pubmed-meshheading:11733503-Cell Line,
pubmed-meshheading:11733503-Cricetinae,
pubmed-meshheading:11733503-DNA Primers,
pubmed-meshheading:11733503-DNA-Binding Proteins,
pubmed-meshheading:11733503-Fibroblast Growth Factor 4,
pubmed-meshheading:11733503-Fibroblast Growth Factors,
pubmed-meshheading:11733503-Fluorescent Antibody Technique,
pubmed-meshheading:11733503-Humans,
pubmed-meshheading:11733503-Nuclear Proteins,
pubmed-meshheading:11733503-Phosphorylation,
pubmed-meshheading:11733503-Promoter Regions, Genetic,
pubmed-meshheading:11733503-Proto-Oncogene Proteins,
pubmed-meshheading:11733503-Subcellular Fractions,
pubmed-meshheading:11733503-Trans-Activators,
pubmed-meshheading:11733503-Transcription, Genetic
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pubmed:year |
2002
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pubmed:articleTitle |
Effects of B-Myb on gene transcription: phosphorylation-dependent activity ans acetylation by p300.
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pubmed:affiliation |
Eppley Institute for Research in Cancer and Allied Diseases, Department of Pathology University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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