Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
2001-12-5
pubmed:databankReference
pubmed:abstractText
We purified microtubules from a mammalian mitotic extract and obtained an amino acid sequence from each microtubule-associated protein by using mass spectrometry. Most of these proteins are known spindle-associated components with essential functional roles in spindle organization. We generated antibodies against a protein identified in this collection and refer to it as astrin because of its association with astral microtubule arrays assembled in vitro. Astrin is approximately 134 kDa, and except for a large predicted coiled-coil domain in its C-terminal region it lacks any known functional motifs. Astrin associates with spindle microtubules as early as prophase where it concentrates at spindle poles. It localizes throughout the spindle in metaphase and anaphase and associates with midzone microtubules in anaphase and telophase. Astrin also localizes to kinetochores but only on those chromosomes that have congressed. Deletion analysis indicates that astrin's primary spindle-targeting domain is at the C terminus, although a secondary domain in the N terminus can target some of the protein to spindle poles. Thus, we have generated a comprehensive list of major mitotic microtubule-associated proteins, among which is astrin, a nonmotor spindle protein.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10373309, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10477750, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10525540, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10747094, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10766876, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10871281, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10966454, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-10993066, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11030625, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11121038, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11146657, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11146661, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11157972, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-11408577, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-1671864, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-1854485, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-2139718, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-2271730, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-2683078, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-7518465, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-7593190, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-7626882, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-7822408, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-7917323, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-8432734, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-8608594, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-8684481, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-8896597, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-9580556, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724960-9989498
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14434-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11724960-Amino Acid Sequence, pubmed-meshheading:11724960-Animals, pubmed-meshheading:11724960-Base Sequence, pubmed-meshheading:11724960-Cell Cycle, pubmed-meshheading:11724960-Cell Cycle Proteins, pubmed-meshheading:11724960-Cell Line, pubmed-meshheading:11724960-DNA, Complementary, pubmed-meshheading:11724960-HeLa Cells, pubmed-meshheading:11724960-Humans, pubmed-meshheading:11724960-Kinetochores, pubmed-meshheading:11724960-Mass Spectrometry, pubmed-meshheading:11724960-Microtubule-Associated Proteins, pubmed-meshheading:11724960-Microtubules, pubmed-meshheading:11724960-Mitosis, pubmed-meshheading:11724960-Mitotic Spindle Apparatus, pubmed-meshheading:11724960-Molecular Sequence Data, pubmed-meshheading:11724960-Recombinant Fusion Proteins, pubmed-meshheading:11724960-Sequence Homology, Amino Acid, pubmed-meshheading:11724960-Transfection
pubmed:year
2001
pubmed:articleTitle
Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein.
pubmed:affiliation
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.