Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-11-1
pubmed:abstractText
A fluorescence polarization-based functional assay for cyclic AMP (cAMP) production in cells has been proven effective for the detection of agonist-stimulated cAMP production in a HEK 293 recombinant cell line expressing the corticotropin-releasing factor subtype 2alpha (CRF2alpha) receptor. Assays were completed in a single well of a 384-well microplate with no transfer, separation, or wash steps incurred. The assay performance is excellent for adaptation to the high throughput screening environment in terms of speed of analysis, magnitude of displaced signal, precision, and detection limits for cAMP quantitation. Relative potencies of agonists and antagonists are maintained with respect to radiometric assays. The assay withstands up to 5% DMSO and up to 10 microM concentrations of highly colored compound. These attributes suggest that accurate assessment of drug binding can be measured using this assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Amphibian Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CRF(2alpha) receptor, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide, http://linkedlifedata.com/resource/pubmed/chemical/Forskolin, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Hormones, http://linkedlifedata.com/resource/pubmed/chemical/Peptides, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Corticotropin-Releasing..., http://linkedlifedata.com/resource/pubmed/chemical/Solvents, http://linkedlifedata.com/resource/pubmed/chemical/Urotensins, http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents, http://linkedlifedata.com/resource/pubmed/chemical/sauvagine
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1087-0571
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-82
pubmed:dateRevised
2011-5-23
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Homogeneous cell-based fluorescence polarization assay for the direct detection of cAMP.
pubmed:affiliation
PerkinElmer Life Sciences, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article