Source:http://linkedlifedata.com/resource/pubmed/id/11686239
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
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| pubmed:dateCreated |
2001-10-31
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| pubmed:abstractText |
Expression of the prespore-specific gene 3B in Dictyostelium discoideum Ax-2 cells is first detectable late in development with 3B mRNA levels peaking at 18 h (Corney et al., 1990). Sequence analysis of 3B cDNA and genomic clones revealed two exons, 319bp and 341bp long, separated by an 82bp intron, which encode a 219 residue protein with no significant similarity to any other reported gene product. Transcription starts at an A residue 45bp upstream from the translation initiation codon, preceded by a TATA-like sequence and an oligo-dT stretch. The 5' flanking sequence of the 3B gene is extremely A + T rich but contains five G/C rich stretches, each approximately 7bp long, which have strong sequence similarity to the G boxes found upstream of other developmentally regulated Dictyostelium genes. Analysis of both 3B promoter-CAT reporter gene and 3B promoter-lacZ reporter gene constructs showed that 908bp of 5' flanking sequence is sufficient to confer correct developmental and cell-type specific regulation. Sequential 5' deletion analysis revealed that positive elements lie upstream of position -304 and that negative element(s) lie between positions -264 and -241. Nevertheless, a 286bp promoter fragment containing only sequence located downstream of position -241 directed essentially correct reporter gene expression. Point mutation analysis identified cis-acting elements within this 'sufficient' promoter fragment which activate transcription (G box V and psp-AT type sequences). A short (56bp) region of the 3B promoter sequence containing both G box IV and the psp-AT type element binds two types of nuclear factor, one present in cells throughout development and a second that appears only in late development with a time course comparable to 3B gene induction.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0301-4681
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
68
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
92-105
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11686239-Amino Acid Sequence,
pubmed-meshheading:11686239-Animals,
pubmed-meshheading:11686239-Base Sequence,
pubmed-meshheading:11686239-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:11686239-Cloning, Molecular,
pubmed-meshheading:11686239-DNA Mutational Analysis,
pubmed-meshheading:11686239-Dictyostelium,
pubmed-meshheading:11686239-Fungal Proteins,
pubmed-meshheading:11686239-Gene Expression Regulation, Developmental,
pubmed-meshheading:11686239-Molecular Sequence Data,
pubmed-meshheading:11686239-Nuclear Proteins,
pubmed-meshheading:11686239-Point Mutation,
pubmed-meshheading:11686239-Promoter Regions, Genetic,
pubmed-meshheading:11686239-Protozoan Proteins,
pubmed-meshheading:11686239-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:11686239-Spores,
pubmed-meshheading:11686239-Transcription Initiation Site,
pubmed-meshheading:11686239-Transcriptional Activation,
pubmed-meshheading:11686239-beta-Galactosidase
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| pubmed:year |
2001
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| pubmed:articleTitle |
Control elements of Dictyostelium discoideum prespore specific gene 3B.
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| pubmed:affiliation |
School of Biochemistry and Molecular Biology, University of Leeds, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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