Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-10-29
pubmed:abstractText
Mycobacterium abscessus and Mycobacterium chelonae are two closely related species that are often not distinguished by clinical laboratories despite the fact they cause diseases requiring different treatment regimens. Multilocus enzyme electrophoresis, PCR-restriction fragment length polymorphism analysis of the 65-kDa heat shock protein gene, biochemical tests, and high-performance liquid chromatography of mycolic acids were used to identify 75 isolates as either M. abscessus or M. chelonae that were originally submitted for drug susceptibility testing. Only 36 of these isolates were submitted with an identification at the species level. Using the above methods, 46 of the isolates were found to be M. abscessus and 29 were identified as M. chelonae. Eight isolates originally submitted as M. chelonae were identified as M. abscessus, and one isolate submitted as M. abscessus was found to be M. chelonae. The four identification methods were in agreement in identifying 74 of the 75 isolates. In drug susceptibility testing, all isolates of M. abscessus exhibited resistance to tobramycin (MIC of 8 to > or =16 microg/ml), while all isolates of M. chelonae were susceptible to this drug (MIC of < or = 4 microg/ml). The results suggest that once an identification method is selected, clinical laboratories should be able to easily identify isolates of M. abscessus and M. chelonae.
pubmed:commentsCorrections
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pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4103-10
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11682537-Anti-Bacterial Agents, pubmed-meshheading:11682537-Bacterial Proteins, pubmed-meshheading:11682537-Bacterial Typing Techniques, pubmed-meshheading:11682537-Chaperonin 60, pubmed-meshheading:11682537-Chaperonins, pubmed-meshheading:11682537-Chromatography, High Pressure Liquid, pubmed-meshheading:11682537-Electrophoresis, Agar Gel, pubmed-meshheading:11682537-Enzymes, pubmed-meshheading:11682537-Humans, pubmed-meshheading:11682537-Microbial Sensitivity Tests, pubmed-meshheading:11682537-Mycobacterium Infections, Nontuberculous, pubmed-meshheading:11682537-Mycobacterium chelonae, pubmed-meshheading:11682537-Mycolic Acids, pubmed-meshheading:11682537-Nontuberculous Mycobacteria, pubmed-meshheading:11682537-Polymerase Chain Reaction, pubmed-meshheading:11682537-Polymorphism, Restriction Fragment Length
pubmed:year
2001
pubmed:articleTitle
Comparison of methods for Identification of Mycobacterium abscessus and M. chelonae isolates.
pubmed:affiliation
Tuberculosis/Mycobacteriology Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. may2@cdc.gov
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies