Linked Life Data

11679409

Source:http://linkedlifedata.com/resource/pubmed/id/11679409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2001-10-26
pubmed:abstractText
Advanced age is associated with endothelial dysfunction and increased risk for atherosclerosis. However, the mechanisms for these observed effects are not clear. To clarify the association between aging and loss of endothelial function, young human aortic endothelial cells (HAECs), senescent HAECs transfected with control vector, and immortalized HAECs containing human telomerase reverse transcriptase (hTERT) were compared for expression of endothelial nitric oxide synthase (eNOS) and production of NO. To investigate a specific function modulated by endothelial NO, adhesion of monocytes under basal conditions as well as after exposure to TNF-alpha was assessed. A decrease in eNOS mRNA, protein, and activity was observed in endothelial cells at senescence as compared with young HAEC; this effect was blunted in hTERT cells. In all cells, shear stress induced a greater increase in the expression of eNOS protein with the final result being higher levels in hTERT compared with senescent cells. Basal monocyte binding was significantly elevated on aged endothelial cells compared with parental and hTERT cells. Exposure of TNF-alpha resulted in a 2-fold increase in monocyte adhesion in senescent cells, whereas this effect was reduced in cells transfected with hTERT. Prior exposure to fluid flow significantly reduced subsequent monocyte adhesion in all groups. These studies demonstrate that replicative aging results in decreased endothelial expression of eNOS accompanied by enhanced monocyte binding. Stable expression of hTERT results in endothelial cells with a younger phenotype with greater amount of eNOS and NO activity. Thus, telomerase transfection may have important functional consequences on endothelial cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
793-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11679409-Aging, pubmed-meshheading:11679409-Aorta, pubmed-meshheading:11679409-Blotting, Western, pubmed-meshheading:11679409-Cell Adhesion, pubmed-meshheading:11679409-Cell Aging, pubmed-meshheading:11679409-Cells, Cultured, pubmed-meshheading:11679409-DNA-Binding Proteins, pubmed-meshheading:11679409-Endothelium, Vascular, pubmed-meshheading:11679409-Humans, pubmed-meshheading:11679409-Monocytes, pubmed-meshheading:11679409-Nitric Oxide, pubmed-meshheading:11679409-Nitric Oxide Synthase, pubmed-meshheading:11679409-Nitric Oxide Synthase Type III, pubmed-meshheading:11679409-RNA, Messenger, pubmed-meshheading:11679409-Stress, Mechanical, pubmed-meshheading:11679409-Telomerase, pubmed-meshheading:11679409-Transfection, pubmed-meshheading:11679409-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
eNOS activity is reduced in senescent human endothelial cells: Preservation by hTERT immortalization.
pubmed:affiliation
Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305-5246, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't