Linked Life Data

11678640

Source:http://linkedlifedata.com/resource/pubmed/id/11678640

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-10-26
pubmed:abstractText
We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but its expression level decreased following stimulation by tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). PECAM-1 was also constitutively expressed on microvascular endothelial cells MvE and GEC, but at lower levels than on HUVEC, and expression by these cells also decreased in response to TNF-alpha and IFN-gamma. There was no dose-dependent effect on MvE but there was a dose-dependent effect on the level of expression of cell adhesion molecules on GEC. TNF-alpha induced the expression of VCAM-1 on HUVEC and GEC, but not MvE, while IFN-gamma induced VCAM-1 expression only on HUVEC. TNF-alpha induced the expression of E-selectin on all three kinds of endothelial cells, but IFN-gamma had no effect on E-selectin expression. GEC therefore showed expression patterns of PECAM-1, VCAM-1, and E-selectin different from those seen in HUVEC and MvE upon treatment with TNF-alpha or IFN-gamma. The use of cultured human GEC allows us to study not only the inflammatory processes, but also the pathophysiological role of GEC in hemodynamic disturbances and their interaction with intrinsic mesangial cells at the molecular and subcellular levels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0026-2862
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-91
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11678640-Antigens, CD31, pubmed-meshheading:11678640-Cell Adhesion Molecules, pubmed-meshheading:11678640-Cell Survival, pubmed-meshheading:11678640-Cells, Cultured, pubmed-meshheading:11678640-Cytokines, pubmed-meshheading:11678640-Dose-Response Relationship, Drug, pubmed-meshheading:11678640-E-Selectin, pubmed-meshheading:11678640-Endothelium, Vascular, pubmed-meshheading:11678640-Humans, pubmed-meshheading:11678640-Intercellular Junctions, pubmed-meshheading:11678640-Interferon-gamma, pubmed-meshheading:11678640-Kidney Glomerulus, pubmed-meshheading:11678640-Membrane Glycoproteins, pubmed-meshheading:11678640-Skin, pubmed-meshheading:11678640-Tumor Necrosis Factor-alpha, pubmed-meshheading:11678640-Umbilical Veins, pubmed-meshheading:11678640-Vascular Cell Adhesion Molecule-1
pubmed:year
2001
pubmed:articleTitle
Expression of adhesion molecules by cultured human glomerular endothelial cells in response to cytokines: comparison to human umbilical vein and dermal microvascular endothelial cells.
pubmed:affiliation
Department of Cellular Physiology, Institute of Nephrology, Niigata University Faculty of Medicine, Niigata 951-8510, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't