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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-10-23
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pubmed:abstractText |
The ATP-induced increase in the cytosolic Ca(2+) concentration ([Ca]i) and current in acutely dissociated ventromedial hypothalamic rats neurons were investigated using fura-2 microfluorometry and the nystatin-perforated patch recording method, respectively. The ATP-induced [Ca]i increase was mimicked by dimethyl-thio-ATP and ATPgammaS, and was inhibited by P2 purinoreceptor antagonists. The ATP-induced [Ca]i increase was markedly reduced by removal of external Na(+) or Ca(2+), and by addition of various Ca(2+) channel antagonists. ATP induced a transient inward current exhibiting a strong inward rectification at membrane potentials more positive than -20 mV. The ATP-induced current at a holding potential of -70 mV was concentration-dependent with a half-maximum effective concentration of 26 microM. Increasing the external Ca(2+) concentration to 10 mM shifted the dose-response relationship to the right. ATP induced only a small current and a small increase in [Ca]i, even at 10 mM Ca(2+), when external Na(+) was removed, suggesting the relatively low permeability to Ca(2+) of purinoceptor channels. These results suggest that ATP activates non-selective cation channels by acting on P2X purinoceptors on dissociated ventromedial hypothalamic neurons, which in turn increases [Ca]i by increasing Ca(2+) influx through voltage-dependent Ca(2+) channels.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Aggregation Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridoxal Phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Suramin,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc,
http://linkedlifedata.com/resource/pubmed/chemical/pyridoxal...
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pubmed:status |
MEDLINE
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pubmed:issn |
0306-4522
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
393-401
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:11672606-Adenosine Triphosphate,
pubmed-meshheading:11672606-Animals,
pubmed-meshheading:11672606-Calcium,
pubmed-meshheading:11672606-Calcium Channels,
pubmed-meshheading:11672606-Cells, Cultured,
pubmed-meshheading:11672606-Cytosol,
pubmed-meshheading:11672606-Dose-Response Relationship, Drug,
pubmed-meshheading:11672606-Extracellular Space,
pubmed-meshheading:11672606-Glutamic Acid,
pubmed-meshheading:11672606-Magnesium,
pubmed-meshheading:11672606-Membrane Potentials,
pubmed-meshheading:11672606-Neurons,
pubmed-meshheading:11672606-Platelet Aggregation Inhibitors,
pubmed-meshheading:11672606-Potassium Chloride,
pubmed-meshheading:11672606-Pyridoxal Phosphate,
pubmed-meshheading:11672606-Rats,
pubmed-meshheading:11672606-Rats, Wistar,
pubmed-meshheading:11672606-Receptors, Purinergic P2,
pubmed-meshheading:11672606-Sodium,
pubmed-meshheading:11672606-Sodium Channels,
pubmed-meshheading:11672606-Suramin,
pubmed-meshheading:11672606-Ventromedial Hypothalamic Nucleus,
pubmed-meshheading:11672606-Zinc
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pubmed:year |
2001
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pubmed:articleTitle |
Excitatory effect of ATP on acutely dissociated ventromedial hypothalamic neurons of the rat.
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pubmed:affiliation |
Department of Physiology, Faculty of Medicine, Kagoshima University, Japan. ciliary@m.kufm.kagoshima-u.ac.jp
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pubmed:publicationType |
Journal Article
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