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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2001-11-5
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pubmed:abstractText |
By screening phage display random peptide libraries with purified immunoglobulin E (IgE) from birch pollen-allergic patients, we previously defined peptides mimicking natural IgE epitopes (mimotopes) of the major birch pollen allergen Bet v 1. The present study aimed to define a monovalent carrier for the IgE mimotopes to induce protective antibodies directed to the IgE epitopes, suitable for mimotope-specific therapy. We expressed the selected mimotopes as fusion proteins together with streptococcal albumin binding protein (ABP). The fusion proteins were recognized specifically by anti-Bet v 1 human IgE, which demonstrated that the mimotopes fused to ABP resemble the natural IgE epitope. Bet v 1-specific IgG was induced by immunization of BALB/c mice with fusion proteins. These IgG antibodies could inhibit IgE binding to Bet v 1. Skin testing of Bet v 1 allergic mice showed that the ABP mimotope constructs did not elicit type I skin reactions, although they possess IgE binding structures. Our data suggest that IgE mimotopes are safe for epitope-specific immunotherapy of sensitized individuals, when presented in a monovalent form. Therefore, ABP-fused mimotopes are promising candidates for a new type of immunotherapy based on the precise induction of blocking antibodies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/Bet v 1 allergen, Betula,
http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Profilins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Albumin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1530-6860
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pubmed:author |
pubmed-author:Barbara Sponer,
pubmed-author:BaumannSS,
pubmed-author:Boltz-NitulescuGG,
pubmed-author:BreitenederHH,
pubmed-author:GanglbergerEE,
pubmed-author:HafnerCC,
pubmed-author:Jensen-JarolimEE,
pubmed-author:SchöllII,
pubmed-author:ScheinerOO,
pubmed-author:SuterMM,
pubmed-author:WiedermannUU
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pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2524-6
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pubmed:dateRevised |
2011-6-21
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pubmed:meshHeading |
pubmed-meshheading:11641259-Allergens,
pubmed-meshheading:11641259-Amino Acid Sequence,
pubmed-meshheading:11641259-Animals,
pubmed-meshheading:11641259-Antibodies,
pubmed-meshheading:11641259-Antibody Formation,
pubmed-meshheading:11641259-Antigens, Plant,
pubmed-meshheading:11641259-Contractile Proteins,
pubmed-meshheading:11641259-Epitopes,
pubmed-meshheading:11641259-Humans,
pubmed-meshheading:11641259-Hypersensitivity,
pubmed-meshheading:11641259-Immunoglobulin E,
pubmed-meshheading:11641259-Immunoglobulin G,
pubmed-meshheading:11641259-Immunotherapy,
pubmed-meshheading:11641259-Mice,
pubmed-meshheading:11641259-Mice, Inbred BALB C,
pubmed-meshheading:11641259-Microfilament Proteins,
pubmed-meshheading:11641259-Molecular Mimicry,
pubmed-meshheading:11641259-Plant Proteins,
pubmed-meshheading:11641259-Plasmids,
pubmed-meshheading:11641259-Profilins,
pubmed-meshheading:11641259-Receptors, Albumin,
pubmed-meshheading:11641259-Recombinant Fusion Proteins,
pubmed-meshheading:11641259-Skin Tests
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pubmed:year |
2001
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pubmed:articleTitle |
Monovalent fusion proteins of IgE mimotopes are safe for therapy of type I allergy.
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pubmed:affiliation |
Department of Pathophysiology, University of Vienna, A-1090 Vienna, Austria.
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pubmed:publicationType |
Journal Article
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