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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2001-10-12
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pubmed:abstractText |
Calcium-dependent protein kinases (CDPKs) comprise a large family of serine/threonine kinases in plants and protozoans. We isolated two related CDPK cDNAs (NtCDPK2 and NtCDPK3) from Nicotiana tabacum. These CDPK transcripts are elevated after race-specific defence elicitation and hypo-osmotic stress. Transiently expressed myc-epitope-tagged NtCDPK2 in Nicotiana benthamiana and N.tabacum leaves showed a rapid transient interconversion to an activated form after elicitation and hypo-osmotic stress. The Avr9 race-specific elicitor caused a more pronounced and sustained response. This transition is due to phosphorylation of the CDPK. Immuno complex kinase assays with epitope-tagged NtCDPK2 showed that stress-induced phosphorylation and interconversion of NtCDPK2 correlates with an increase in enzymatic activity. The function of NtCDPK2 in plant defence was investigated by employing virus-induced gene silencing (VIGS) in N.benthamiana. CDPK-silenced plants showed a reduced and delayed hypersensitive response after race-specific elicitation in a gene-for-gene interaction, and lacked an accompanying wilting phenotype. Silencing correlated with loss of CDPK mRNA, whereas mRNA accumulation of mitogen-activated protein kinase WIPK remained unaltered.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10066609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10066637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10080688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10322199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10344204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10486561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10590159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10656593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10717008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10740296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10747788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10758503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10810144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10810151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10823962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10830163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10838614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10852940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10890528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-10929125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11069701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11090222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11115124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11123804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11158543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11199391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-11495791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-1338696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-7599646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-7916621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8003490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8078458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8220456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8755249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8755618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8756605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8855961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8943201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-8978683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9013887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9224713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9421928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9688575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9691114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9927643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11597999-9927644
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AVR4 protein, Cladosporium fulvum,
http://linkedlifedata.com/resource/pubmed/chemical/AVR9 protein, Cladosporium fulvum,
http://linkedlifedata.com/resource/pubmed/chemical/CDPK3 protein, Plasmodium falciparum,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cf protein, Lycopersicon esculentum,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/WIPK protein, Nicotiana tabacum
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5556-67
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11597999-Tobacco,
pubmed-meshheading:11597999-Phosphorylation,
pubmed-meshheading:11597999-Plant Leaves,
pubmed-meshheading:11597999-Fungal Proteins,
pubmed-meshheading:11597999-Plant Proteins,
pubmed-meshheading:11597999-Cells, Cultured,
pubmed-meshheading:11597999-RNA, Messenger,
pubmed-meshheading:11597999-Amino Acid Sequence,
pubmed-meshheading:11597999-Phenotype,
pubmed-meshheading:11597999-Osmotic Pressure,
pubmed-meshheading:11597999-Enzyme Induction
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