Source:http://linkedlifedata.com/resource/pubmed/id/11595752
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-10-11
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| pubmed:abstractText |
To investigate the existence of 5'-region(s) of human choline acetyltransferase (hChAT) mRNA in placenta we analyzed the presence or absence of ChAT 5'-untranslated regions (UTR) in human neuronal and non-neuronal cells. Total RNA from human spinal cord, placenta, cultured choriocarcinoma JEG-3 and neuroblastoma CHP126 and MC-IXC cells was reverse transcribed and used for polymerase chain reaction amplification (RT-PCR). We used a sense primer located in the 5'-flanking region, in the previously defined intronic sequence and an anti-sense primer located in the common coding exon 2 of the hChAT gene. An amplified product of 567 bp in size was obtained only in human placenta and in JEG-3 cells whereas it was absent in spinal cord, CHP126 and MC-IXC cells. It was designated 'H-type' of ChAT mRNA. Whereas CHP126 produced the R- and N-type of ChAT mRNAs, no transcript of the N-and R-type was detected in JEG-3 and human placenta. In addition, CHP126 and JEG-3 cells and placenta showed the expression of the M-type of ChAT mRNA. The identity of the amplified 567 bp product (H-type) was confirmed by Southern hybridization and sequencing. The nucleotide sequence of the amplified fragment in placenta revealed the existence of a previously unknown type of ChAT mRNA produced by alternative splicing. Using primer extension we further determined the transcription initiation site of the H-type hChAT mRNA in placenta. These results demonstrate the expression of a novel ChAT mRNA isoform in human placenta in addition to the M-type. These data may be possibly explained by the presence of a placenta specific promoter in the ChAT gene, which might be the proximal promoter P1.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
79
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
9-16
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11595752-Amino Acid Sequence,
pubmed-meshheading:11595752-Base Sequence,
pubmed-meshheading:11595752-Blotting, Southern,
pubmed-meshheading:11595752-Choline O-Acetyltransferase,
pubmed-meshheading:11595752-Choriocarcinoma,
pubmed-meshheading:11595752-Exons,
pubmed-meshheading:11595752-Female,
pubmed-meshheading:11595752-Humans,
pubmed-meshheading:11595752-Molecular Sequence Data,
pubmed-meshheading:11595752-Neuroblastoma,
pubmed-meshheading:11595752-Placenta,
pubmed-meshheading:11595752-Pregnancy,
pubmed-meshheading:11595752-RNA, Messenger,
pubmed-meshheading:11595752-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11595752-Spinal Cord,
pubmed-meshheading:11595752-Trophoblasts,
pubmed-meshheading:11595752-Tumor Cells, Cultured,
pubmed-meshheading:11595752-Uterine Neoplasms
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
A novel untranslated 'exon H' of the human choline acetyltransferase gene in placenta.
|
| pubmed:affiliation |
Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, Biologie Moléculaire et de Génie Génétique, INSERM, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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