Source:http://linkedlifedata.com/resource/pubmed/id/11577715
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
|
pubmed:dateCreated |
2001-10-1
|
pubmed:abstractText |
The soil bacterium Bacillus cereus Tim-r01 efficiently transformed polyaromatic carboxylic acids (PACA) such as 4-biphenylcarboxylic acid (4-BPCA), 4-biphenylacetic acid, and 4-phenoxybenzoic acid into their corresponding amides. The amidation activity was expressed at 37 degrees C (pH 7-8) in the presence of grown cells in nutrients under an aerobic atmosphere. Other strains of B. cereus, IFO 3001 and IAM 1229, also gave the amide from 4-BPCA. In phosphate-buffered saline (PBS), the addition of normal amino acids was essential, while sulfur-containing amino acids such as methionine and cysteine drastically inhibited the amidation. Tracer experiments using N-15-isoleucine and N-15-alanine showed that the nitrogen atom of the amide came from an amino group of amino acids but not from ammonia or alkylamines.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carboxylic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Polycyclic Hydrocarbons, Aromatic
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0916-8451
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
65
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1761-5
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11577715-Amides,
pubmed-meshheading:11577715-Amino Acids,
pubmed-meshheading:11577715-Bacillus cereus,
pubmed-meshheading:11577715-Carboxylic Acids,
pubmed-meshheading:11577715-Culture Media,
pubmed-meshheading:11577715-Indicators and Reagents,
pubmed-meshheading:11577715-Magnetic Resonance Spectroscopy,
pubmed-meshheading:11577715-Nitrogen,
pubmed-meshheading:11577715-Polycyclic Hydrocarbons, Aromatic
|
pubmed:year |
2001
|
pubmed:articleTitle |
Effects of amino acids on the amidation of polyaromatic carboxylic acids by Bacillus cereus.
|
pubmed:affiliation |
Department of Cell Engineering, Faculty of Engineering, Toyama University, Gofuku, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|