Linked Life Data

11567647

Source:http://linkedlifedata.com/resource/pubmed/id/11567647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-9-24
pubmed:abstractText
Immunosuppressants are now known to modulate bone metabolism, including bone formation and resorption. Because cartilage, formed by differentiated chondrocytes, serves as a template for endochondral bone formation, we examined the effects of the immunosuppressant rapamycin on the chondrogenesis of mesenchymal cells and on the cell signaling that is required for chondrogenesis, such as protein kinase C, extracellular signal-regulated kinase-1 (ERK-1), and p38 mitogen-activated protein (MAP) kinase pathways. Rapamycin inhibited the expression of type II collagen and the accumulation of sulfate glycosaminoglycan, indicating inhibition of the chondrogenesis of mesenchymal cells. Rapamycin treatment did not affect precartilage condensation, but it prevented cartilage nodule formation. Exposure of chondrifying mesenchymal cells to rapamycin blocked activation of the protein kinase C alpha and p38 MAP kinase, but had no discernible effect on ERK-1 signaling. Selective inhibition of PKCalpha or p38 MAP kinase activity, which is dramatically increased during chondrogenesis, with specific inhibitors in the absence of rapamycin blocked the chondrogenic differentiation of mesenchymal cells. Taken together, our data indicate that the immunosuppressant rapamycin inhibits the chondrogenesis of mesenchymal cells at the post-precartilage condensation stage by modulating signaling pathways including those of PKCalpha and p38 MAP kinase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
427
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11567647-Animals, pubmed-meshheading:11567647-Cartilage, pubmed-meshheading:11567647-Cell Culture Techniques, pubmed-meshheading:11567647-Cell Division, pubmed-meshheading:11567647-Cells, Cultured, pubmed-meshheading:11567647-Chick Embryo, pubmed-meshheading:11567647-Chondrogenesis, pubmed-meshheading:11567647-Enzyme Activation, pubmed-meshheading:11567647-Immunosuppressive Agents, pubmed-meshheading:11567647-Isoenzymes, pubmed-meshheading:11567647-Mesoderm, pubmed-meshheading:11567647-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:11567647-Mitogen-Activated Protein Kinases, pubmed-meshheading:11567647-Phosphorylation, pubmed-meshheading:11567647-Protein Kinase C, pubmed-meshheading:11567647-Protein Kinase C-alpha, pubmed-meshheading:11567647-Ribosomal Protein S6 Kinases, pubmed-meshheading:11567647-Sirolimus, pubmed-meshheading:11567647-p38 Mitogen-Activated Protein Kinases
pubmed:year
2001
pubmed:articleTitle
Immunosuppressant rapamycin inhibits protein kinase C alpha and p38 mitogen-activated protein kinase leading to the inhibition of chondrogenesis.
pubmed:affiliation
National Research Laboratory, Department of Life Science, Kwangju Institute of Science and Technology, Buk-Gu, Kwangju 500-712, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't