Linked Life Data

11563062

Source:http://linkedlifedata.com/resource/pubmed/id/11563062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-7
pubmed:dateCreated
2001-9-20
pubmed:abstractText
The HIV-1 Tat protein interaction with its RNA recognition sequence TAR is an important drug target and model system for the development of specific RNA-protein inhibitors. 2'-O-methyl oligoribonucleotides complementary to the TAR apical stem-loop effectively block Tat binding in vitro. Substitution by 5-propynylC or 5-methylC LNA monomeric units into a 12-mer 2'-O-methyl oligoribonucleotide leads to stronger inhibition, as does a 12-mer PNA. 10-16 mer 2'-O-methyl oligoribonucleotides give sequence- and dose-dependent inhibition of Tat-dependent transcription of an HIV DNA template in HeLa cell nuclear extract. Inhibition is maintained for the substituted 12-mer analogues but is poorer for PNA and is not correlated with TAR binding strength.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1525-7770
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
471-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Oligonucleotide analogue interference with the HIV-1 Tat protein-TAR RNA interaction.
pubmed:affiliation
Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't