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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2001-9-17
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pubmed:abstractText |
1. Whole-cell current responses to brief flashes were obtained from voltage-clamped 'on' bipolar cells in dark-adapted dogfish retinal slices. When internal Ca2+ was buffered to low levels, the current-voltage (I-V) relation of their flash responses was linear, with a reversal potential near 0 mV. 2. On elevating internal Ca2+ the light-dependent I-V relation showed outward rectification, such that the current response to a flash decreased e-fold for a hyperpolarization of 22 mV. 3. Inclusion of a CaMKII inhibitory peptide in the patch-pipette solution removed the rectification even in the presence of 50 microM Ca2+. 4. These results are consistent with CaMKII phosphorylation of cGMP-activated channels leading to a voltage-dependent reduction in conductance (outward rectification) and a reduced light response. The voltage-dependent property suggests that phosphorylation creates an energy barrier near the outer part of the channel, reducing the flow principally of monovalent cations. 5. This is the first reported instance of CaMKII phosphorylation acting to change the electrical characteristics of a membrane channel from linear to rectifying. 6. Ca2+-dependent desensitization by background light and channel rectification may underlie the change in centre-surround organization of the visual system with light adaptation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10349971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10366613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10828243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10839540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10844016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-10879537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-11034622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-13035466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-13463771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-1348117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-1358250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-1382474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-1515915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-1695713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-4747229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-7381782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-7538564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-7626114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-7841125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-8389366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11559768-9852318
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
535
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-702
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11559768-Adaptation, Ocular,
pubmed-meshheading:11559768-Algorithms,
pubmed-meshheading:11559768-Animals,
pubmed-meshheading:11559768-Calcium,
pubmed-meshheading:11559768-Calcium-Calmodulin-Dependent Protein Kinase Type 2,
pubmed-meshheading:11559768-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:11559768-Cell Membrane,
pubmed-meshheading:11559768-Cell Polarity,
pubmed-meshheading:11559768-Cyclic GMP,
pubmed-meshheading:11559768-Dogfish,
pubmed-meshheading:11559768-Electrophysiology,
pubmed-meshheading:11559768-Ion Channels,
pubmed-meshheading:11559768-Patch-Clamp Techniques,
pubmed-meshheading:11559768-Phosphorylation,
pubmed-meshheading:11559768-Photic Stimulation,
pubmed-meshheading:11559768-Receptors, Metabotropic Glutamate,
pubmed-meshheading:11559768-Retina
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pubmed:year |
2001
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pubmed:articleTitle |
Rectification of cGMP-activated channels induced by phosphorylation in dogfish retinal 'on' bipolar cells.
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pubmed:affiliation |
Biophysics Unit, Physiology Department, University College London, Gower Street, London WC1E 6BT, UK. r.shiells@ucl.ac.uk
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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