Linked Life Data

11557026

Source:http://linkedlifedata.com/resource/pubmed/id/11557026

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-9-14
pubmed:abstractText
Poloxamer 407 was adsorbed onto the surface of model colloidal drug carriers, polystyrene nanoparticles of 40, 70 and 137 nm in diameter, and the effect of the degree of surface coverage and the conformation of the poly(ethylene oxide) (PEO) chains on biological fate was studied. The relationship between the physicochemical and the biological properties of the nanoparticle systems was also investigated. The adsorbed layer of poloxamer 407 was characterised in terms of percentage surface coverage, thickness of the adsorbed layer and average surface area per PEO chain. Computer modelling of the adsorbed layer was performed (applying the self-consistent field technique), to obtain the structural information of the PEO chains in the layer. The in vitro interaction of the nanoparticles with different degrees of poloxamer 407 surface coverage with serum components and the in vivo biodistribution in the rat model were assessed. The results demonstrated that an increase in the surface coverage with poloxamer 407 resulted in an increased volume fraction of the PEO in the adsorbed layer, further extension of the PEO chains from the surface and closer packing of the chains at the surface. With regard to the interaction with the serum components, an increased surface coverage resulted in a reduction of the amount of serum proteins adsorbed, and, importantly, affected the type of proteins adsorbed. High molecular weight proteins were not adsorbed onto the nanoparticles with a surface coverage above approx. 25%. Following the intravenous administration to rats, even the nanoparticles with the lowest degree of surface coverage (approx. 5%) showed improved circulation profiles relative to the uncoated nanoparticles. The effect was more pronounced for the 40 nm nanoparticles. A further increase in the surface coverage to approx. 25% resulted in a significant increase in circulation time, as compared to uncoated and 5% coated systems, for all sizes of nanoparticles. Importantly, it was found that a long in vivo blood circulation time could be achieved for nanoparticles with a relatively low degree of surface coverage with PEO chains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
1514
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-79
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11557026-Adsorption, pubmed-meshheading:11557026-Animals, pubmed-meshheading:11557026-Biodegradation, Environmental, pubmed-meshheading:11557026-Blood Proteins, pubmed-meshheading:11557026-Colloids, pubmed-meshheading:11557026-Computer Simulation, pubmed-meshheading:11557026-Drug Carriers, pubmed-meshheading:11557026-Microspheres, pubmed-meshheading:11557026-Molecular Conformation, pubmed-meshheading:11557026-Particle Size, pubmed-meshheading:11557026-Poloxamer, pubmed-meshheading:11557026-Polyethylene Glycols, pubmed-meshheading:11557026-Polystyrenes, pubmed-meshheading:11557026-Propylene Glycols, pubmed-meshheading:11557026-Rats, pubmed-meshheading:11557026-Surface Properties, pubmed-meshheading:11557026-Surface-Active Agents, pubmed-meshheading:11557026-Tissue Distribution
pubmed:year
2001
pubmed:articleTitle
The effect of surface coverage and conformation of poly(ethylene oxide) (PEO) chains of poloxamer 407 on the biological fate of model colloidal drug carriers.
pubmed:affiliation
Institute of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
pubmed:publicationType
Journal Article, In Vitro