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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2001-9-3
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pubmed:abstractText |
1. The continual use of warfarin as a rodenticide has caused the development of populations of warfarin-resistant roof rat. To study the biochemical mechanism of warfarin resistance, the mRNA expression levels of the major P450 forms in the warfarin-resistant and -susceptible roof rat liver following exposure to warfarin were quantified by competitive RT-PCR. 2. The constitutive levels of CYP2C11 and CYP3A2 mRNAs in the warfarin-resistant and -susceptible roof rat were extremely low compared with those in the STD rat. In response to warfarin administration, the CYP3A2 mRNA level in the warfarin-susceptible rat increased to about 3-fold of that before the treatment, whereas in the warfarin-resistant roof rat, CYP3A2 mRNA remained at a low level. 3. The present results suggest the possibility that reduced synthesis of CYP3A2 mRNA is involved in the warfarin-resistant mechanism in the roof rat.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aryl Hydrocarbon Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/CYP2C11 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyp3a2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP3A,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Rodenticides,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid 16-alpha-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/Warfarin,
http://linkedlifedata.com/resource/pubmed/chemical/steroid hormone 6-beta-hydroxylase
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0049-8254
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-407
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11531004-Animals,
pubmed-meshheading:11531004-Aryl Hydrocarbon Hydroxylases,
pubmed-meshheading:11531004-Cytochrome P-450 CYP3A,
pubmed-meshheading:11531004-Cytochrome P-450 Enzyme System,
pubmed-meshheading:11531004-Drug Resistance,
pubmed-meshheading:11531004-Liver,
pubmed-meshheading:11531004-Male,
pubmed-meshheading:11531004-Membrane Proteins,
pubmed-meshheading:11531004-RNA, Messenger,
pubmed-meshheading:11531004-Rats,
pubmed-meshheading:11531004-Rats, Sprague-Dawley,
pubmed-meshheading:11531004-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11531004-Rodenticides,
pubmed-meshheading:11531004-Species Specificity,
pubmed-meshheading:11531004-Steroid 16-alpha-Hydroxylase,
pubmed-meshheading:11531004-Steroid Hydroxylases,
pubmed-meshheading:11531004-Warfarin
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pubmed:year |
2001
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pubmed:articleTitle |
Suppression of CYP3A2 mRNA expression in the warfarin-resistant roof rat, Rattus rattus: possible involvement of cytochrome P450 in the warfarin resistance mechanism.
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pubmed:affiliation |
Department of Biochemistry, School of Nursing, Kitasato University, Sagamihara, Kanagawa, Japan. sugano@kitasato-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study
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