Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2001-8-29
pubmed:abstractText
Ser-Arg-rich (SR) proteins play numerous roles in spliceosome assembly and the regulation of splice-site selection. Whereas considerable attention has focused on the mechanistic details of SR protein activities, little is known concerning how these splicing regulators are controlled by the cell. Here we examined the subcellular localization of precursor mRNA splicing factors during early development of the nematode Ascaris lumbricoides. In the early embryo, before major zygotic gene activation, most SR proteins, along with RNA polymerase II, are localized in the cytoplasm. As development proceeds, we observe a significant decrease in the cytoplasmic levels of these factors and a concomitant increase in nuclear localization. In contrast, trimethylguanosine-capped small nuclear ribonucleoproteins are predominantly localized in the nucleus throughout this period. We previously showed that the phosphorylation state and activity of SR proteins are regulated during A. lumbricoides embryogenesis. These changes correlate with the onset of precursor mRNA splicing and zygotic transcription. Thus, a coordinate change in the subcellular localization of SR proteins and RNA polymerase II occurs at the transition from reliance on maternally deposited factors to embryonic expression. We propose that before zygotic gene activation, SR proteins and RNA polymerase II are stockpiled in the cytoplasm of early embryos, awaiting signals that lead to their activation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10077565, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10196197, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10225947, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10318902, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10320381, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10346811, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10366588, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10385619, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10394358, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10411135, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10490636, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10713112, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10859161, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10952997, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-10999598, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-11233987, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-11336712, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-1571156, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-1695321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-1717489, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-1833187, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2146003, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2471658, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2531654, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2534076, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2967782, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-2996774, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-7526381, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-7536746, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-7585254, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-7673179, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-7988565, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8026638, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8085156, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8139654, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8261509, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8385799, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-8617202, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9008700, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9030686, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9037021, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9159396, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9166400, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9321404, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9404896, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9420331, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9443969, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9640532, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9786943, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526235-9799243
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10184-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Regulation of SR protein localization during development.
pubmed:affiliation
Center for RNA Molecular Biology, Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.